ArticleAmerican journal of translational research2023
Gene set enrichment analysis and ingenuity pathway analysis to verify the impact of Wnt signaling in psoriasis treated with Taodan granules.
Article in American journal of translational research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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Who cites it
2 citing papers in PubMed.
- CRISPR/Cas9-Mediated Knockout and Overexpression Studies Unveil the Role of PD-L1 in Immune Modulation in a Psoriasis-like Mouse Model.Inflammation · 2025Article
- Shared Genetic Characteristics of Coronary Artery Disease and Peripheral artery Disease: Insights From Integrated Bioinformatics Analysis of RNA-Sequencing Data.Bioinformatics and biology insights · 2025Article
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Authors and funding
14 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTaodan granules (TDGs), traditional Chinese herbals, have effectiveness in relieving skin erythema, scales, and other symptoms of psoriasis. Yet mechanisms of TDGs remain indistinct.
objectiveTo indicate the molecular mechanisms of TDGs in treating psoriasis. MATERIALS AND
methodsPrimarily, transcriptional profiling was applied to identify differentially expressed genes (DEGs), proceeding with Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Gene Set Enrichment Analysis (GSEA) and Ingenuity Pathway Analysis (IPA) analysis were used for functional enrichment analysis. Subsequently, levels of selected genes were validated by RT-PCR and western blotting.
resultsThe GSEA results revealed TDGs could down-regulate the Wnt signaling pathway to ameliorate skin lesions of imiquimod (IMQ)-induced psoriatic models mice. IPA core network associated with Wnt signaling pathways in TDGs for psoriasis was established. Thereinto zeste homolog 2 (EZH2), CTNNB1, tumor protein p63 (TP63), and WD repeat domain 5 (WDR5) were considered as upstream genes in the Wnt signaling pathway. Experimental verification indicated TDGs could down-regulate EZH2, CTNNB1, and WDR5 at the mRNA and protein levels, along with up-regulate TP63 levels. Moreover, TDGs were confirmed to reduce RAC2 and WNT5A at mRNA and protein levels of the Wnt signaling pathway.
conclusionsTDGs may improve psoriasis through the regulation for upstream genes (down-regulating levels of EZH2, CTNNB1, and WDR5; up-regulating TP63 levels) of Wnt signaling pathway, thus reducing levels of RAC2 and WNT5A in the Wnt signaling pathway.
Indexed as
Identifiers
36777818PMC9908442What OpenQuestion holds
Registered trials
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