Evidence map›Paper›PMID 36771013›Full record

ArticleMolecules (Basel, Switzerland)2023

Guidelines for the Simulations of Nitroxide X-Band cw EPR Spectra from Site-Directed Spin Labeling Experiments Using S

Emilien Etienne, Annalisa Pierro, Ketty C Tamburrini, Alessio Bonucci, Elisabetta Mileo, Marlène Martinho, Valérie Belle

Abstract read
In one paragraph

Article in Molecules (Basel, Switzerland), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Enhanced Resolution in EPR Spectroscopy Using para-Hydrogen Matrices.Angewandte Chemie (International ed. in English) · 2026
    Article
  5. Article
  6. Review
  7. Article
  8. Review
  9. Article
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Emilien EtienneAix Marseille University, CNRS (Centre National de la Recherche Scientifique), BIP (Bioénergétique et Ingénierie des Protéines), IMM (Institut de Microbiologie de la Méditerranée), 13009 Marseille, France.ORCID 0000-0002-5999-7904
Annalisa PierroAix Marseille University, CNRS (Centre National de la Recherche Scientifique), BIP (Bioénergétique et Ingénierie des Protéines), IMM (Institut de Microbiologie de la Méditerranée), 13009 Marseille, France.ORCID 0000-0002-2892-2168
Ketty C TamburriniAix Marseille University, CNRS (Centre National de la Recherche Scientifique), BIP (Bioénergétique et Ingénierie des Protéines), IMM (Institut de Microbiologie de la Méditerranée), 13009 Marseille, France.
Alessio BonucciAix Marseille University, CNRS (Centre National de la Recherche Scientifique), BIP (Bioénergétique et Ingénierie des Protéines), IMM (Institut de Microbiologie de la Méditerranée), 13009 Marseille, France.
Elisabetta MileoAix Marseille University, CNRS (Centre National de la Recherche Scientifique), BIP (Bioénergétique et Ingénierie des Protéines), IMM (Institut de Microbiologie de la Méditerranée), 13009 Marseille, France.ORCID 0000-0002-0883-6043
Marlène MartinhoAix Marseille University, CNRS (Centre National de la Recherche Scientifique), BIP (Bioénergétique et Ingénierie des Protéines), IMM (Institut de Microbiologie de la Méditerranée), 13009 Marseille, France.ORCID 0000-0001-8576-5675
Valérie BelleAix Marseille University, CNRS (Centre National de la Recherche Scientifique), BIP (Bioénergétique et Ingénierie des Protéines), IMM (Institut de Microbiologie de la Méditerranée), 13009 Marseille, France.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Site-directed spin labeling (SDSL) combined with continuous wave electron paramagnetic resonance (cw EPR) spectroscopy is a powerful technique to reveal, at the local level, the dynamics of structural transitions in proteins. Here, we consider SDSL-EPR based on the selective grafting of a nitroxide on the protein under study, followed by X-band cw EPR analysis. To extract valuable quantitative information from SDSL-EPR spectra and thus give a reliable interpretation on biological system dynamics, a numerical simulation of the spectra is required. However, regardless of the numerical tool chosen to perform such simulations, the number of parameters is often too high to provide unambiguous results. In this study, we have chosen

Indexed as

Nitrogen OxidesProteinsElectron Spin Resonance SpectroscopySpin LabelsNitrogen OxidesnitroxylProteinsSpin LabelsEasySpinEPRESRnitroxideSimLabelsimulationsite-directed spin labeling

Identifiers

PMID36771013
PMCPMC9919594

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.