Evidence map›Paper›PMID 36769376›Full record

ArticleInternational journal of molecular sciences2023

Selection of Novel Reference Genes by RNA-Seq and Their Evaluation for Normalising Real-Time qPCR Expression Data of Anthocyanin-Related Genes in Lettuce and Wild Relatives.

Inés Medina-Lozano, María Soledad Arnedo, Jérôme Grimplet, Aurora Díaz

Open access · goldAbstract read
In one paragraph

Article in International journal of molecular sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Biomedicines · 2024
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Inés Medina-LozanoDepartment of Plant Sciences, Agrifood Research and Technology Centre of Aragon (CITA), Avd. Montañana 930, 50059 Zaragoza, Spain.ORCID 0000-0001-5533-3505
María Soledad ArnedoRamiro Arnedo S.A. Paraje La Molina 54, Las Norias de Daza, 04716 Almería, Spain.
Jérôme GrimpletDepartment of Plant Sciences, Agrifood Research and Technology Centre of Aragon (CITA), Avd. Montañana 930, 50059 Zaragoza, Spain.ORCID 0000-0002-3265-4012
Aurora DíazDepartment of Plant Sciences, Agrifood Research and Technology Centre of Aragon (CITA), Avd. Montañana 930, 50059 Zaragoza, Spain.ORCID 0000-0001-7297-1699
Universidad de Zaragoza · ES

Funding

Gobierno de Aragón A12-17R: "Grupo de investigación en fruticultura: caracterización, adaptación y mejora genética"Gobierno de Aragón LMP148_21Gobierno de Aragón LMP164_18Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria RTA2017-00093-00-00Spanish Ministry of Science, Innovation and Universities (MCIU) and the Spanish State Research Agency (AEI) I. Medina-Lozano predoctoral contractUniversity of Zaragoza APC
6 · The paper itself

Abstract

Lettuce is a popular vegetable source of bioactive compounds, like anthocyanins, powerful antioxidants present in red and semi-red varieties. Selection of reliable reference genes (RGs) for the normalization of real-time quantitative PCR (qPCR) data is crucial to obtain accurate gene expression results. Among the genes with totally unrelated biological functions, six candidate RGs (

Indexed as

AnthocyaninsLactucaGene Expression ProfilingPlant LeavesReal-Time Polymerase Chain ReactionReference StandardsRNA-SeqAnthocyaninsBestKeeperDelta Ct methoddrought stressgeNormLactuca sativa L.leaf colourNormFinderRefFindertissuestranscriptomics

Identifiers

PMID36769376
PMCPMC9917471
OpenAlexW4319318849

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.