Evidence map›Paper›PMID 36769321›Full record

ArticleInternational journal of molecular sciences2023

Protocol for Cell Colonization and Comprehensive Monitoring of Osteogenic Differentiation in 3D Scaffolds Using Biochemical Assays and Multiphoton Imaging.

Kai Peter Sommer, Adrian Krolinski, Mohammad Mirkhalaf, Hala Zreiqat, Oliver Friedrich, Martin Vielreicher

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Kai Peter SommerInstitute of Medical Biotechnology, Department of Chemical and Biological Engineering, Friedrich-Alexander University of Erlangen-Nürnberg, D-91052 Erlangen, Germany.
Adrian KrolinskiInstitute of Medical Biotechnology, Department of Chemical and Biological Engineering, Friedrich-Alexander University of Erlangen-Nürnberg, D-91052 Erlangen, Germany.
Mohammad MirkhalafSchool of Mechanical, Medical and Process Engineering, Queensland University of Technology, 2 George St., Brisbane, QLD 4000, Australia.
Hala ZreiqatBiomaterials and Tissue Engineering Research Unit, School of Aerospace, Mechanical and Mechatronic Engineering, University of Sydney, Sydney, NSW 2006, Australia.
Oliver FriedrichInstitute of Medical Biotechnology, Department of Chemical and Biological Engineering, Friedrich-Alexander University of Erlangen-Nürnberg, D-91052 Erlangen, Germany.ORCID 0000-0003-2238-2049
Martin VielreicherInstitute of Medical Biotechnology, Department of Chemical and Biological Engineering, Friedrich-Alexander University of Erlangen-Nürnberg, D-91052 Erlangen, Germany.ORCID 0000-0002-7515-1431

Funding

Australian Research Council DE210100975Deutsche Forschungsgemeinschaft 2993/25-1Deutsche Forschungsgemeinschaft SFB/TRR 225 (subproject B08)Deutsche Forschungsgemeinschaft VI 770/3-1
6 · The paper itself

Abstract

The goal of bone tissue engineering is to build artificial bone tissue with properties that closely resemble human bone and thereby support the optimal integration of the constructs (biografts) into the body. The development of tissues in 3D scaffolds includes several complex steps that need to be optimized and monitored. In particular, cell-material interaction during seeding, cell proliferation and cell differentiation within the scaffold pores play a key role. In this work, we seeded two types of 3D-printed scaffolds with pre-osteoblastic MC3T3-E1 cells, proliferated and differentiated the cells, before testing and adapting different assays and imaging methods to monitor these processes. Alpha-TCP/HA (α-TCP with low calcium hydroxyapatite) and baghdadite (Ca

Indexed as

OsteogenesisTissue ScaffoldsCalcium PhosphatesCell DifferentiationCell ProliferationCeramicsCollagenDurapatiteHumansSilicatesTissue Engineeringalpha-tricalcium phosphateCa(3)ZrSi(2)O(9)Calcium PhosphatesCollagenDurapatiteSilicates3D-printed scaffoldsbiochemical assaysbiograftbone cellscollagen-Iimagingmineralizationosteogenesis

Identifiers

PMID36769321
PMCPMC9917811

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.