Single-Molecule Localization Microscopy of Presynaptic Active Zones in

Achmed Mrestani, Katharina Lichter, Anna-Leena Sirén, Manfred Heckmann, Mila M Paul, Martin Pauli

Abstract read

In one paragraph

Article in International journal of molecular sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Elucidating the Molecular Mechanism of 3D1 Antibody Binding to a Swine Enteric Coronavirus Antigen.Viruses · 2026
Article

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5 · Who and what money

Authors and funding

6 authors.

Achmed MrestaniDepartment of Neurophysiology, Institute for Physiology, University of Würzburg, 97070 Würzburg, Germany.ORCID 0000-0003-3063-164X

Katharina LichterDepartment of Neurophysiology, Institute for Physiology, University of Würzburg, 97070 Würzburg, Germany.

Anna-Leena SirénDepartment of Neurophysiology, Institute for Physiology, University of Würzburg, 97070 Würzburg, Germany.ORCID 0000-0002-2217-0081

Manfred HeckmannDepartment of Neurophysiology, Institute for Physiology, University of Würzburg, 97070 Würzburg, Germany.

Mila M PaulDepartment of Neurophysiology, Institute for Physiology, University of Würzburg, 97070 Würzburg, Germany.

Martin PauliDepartment of Neurophysiology, Institute for Physiology, University of Würzburg, 97070 Würzburg, Germany.

Funding

Deutsche Forschungsgemeinschaft CRC 166, Project B06 (MH, A-L S), FOR 3004 SYNABS P1 (M.H.)Interdisciplinary Clinical Research Center (IZKF) Würzburg Z-3/69 to M.M.P., N-229 to M.H. and A.-L.S

6 · The paper itself

Abstract

Single-molecule localization microscopy (SMLM) greatly advances structural studies of diverse biological tissues. For example, presynaptic active zone (AZ) nanotopology is resolved in increasing detail. Immunofluorescence imaging of AZ proteins usually relies on epitope preservation using aldehyde-based immunocompetent fixation. Cryofixation techniques, such as high-pressure freezing (HPF) and freeze substitution (FS), are widely used for ultrastructural studies of presynaptic architecture in electron microscopy (EM). HPF/FS demonstrated nearer-to-native preservation of AZ ultrastructure, e.g., by facilitating single filamentous structures. Here, we present a protocol combining the advantages of HPF/FS and

Indexed as

Drosophila melanogasterDrosophila ProteinsAnimalsCryopreservationMicroscopy, ElectronNeuromuscular JunctionSynapsesDrosophila Proteinsactive zoneDrosophila melanogasterdSTORMhigh-pressure freezing/freeze substitutionnanotopologyneuromuscular junctionPFA in ethanol

Identifiers

PMID36768451

DOI10.3390/ijms24032128

PMCPMC9917252

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.