Evidence map›Paper›PMID 36765990›Full record

ArticleFoods (Basel, Switzerland)2023

Characterization of the Technofunctional Properties and Three-Dimensional Structure Prediction of 11S Globulins from Amaranth (

Jorge Aguilar-Padilla, Sara Centeno-Leija, Esaú Bojórquez-Velázquez, José M Elizalde-Contreras, Eliel Ruiz-May, Hugo Serrano-Posada, Juan Alberto Osuna-Castro

Abstract read
In one paragraph

Article in Foods (Basel, Switzerland), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Jorge Aguilar-PadillaFacultad de Ciencias Químicas, Universidad de Colima, Carr. Colima-Coquimatlán km. 9, Coquimatlán 28400, Colima, Mexico.ORCID 0000-0003-3070-9945
Sara Centeno-LeijaConsejo Nacional de Ciencia y Tecnología, Laboratorio de Biología Sintética, Estructural y Molecular, Laboratorio de Agrobiotecnología, Tecnoparque CLQ, Universidad de Colima, Carretera Los Limones-Loma de Juárez, Colima 28629, Colima, Mexico.ORCID 0000-0002-8573-4577
Esaú Bojórquez-VelázquezRed de Estudios Moleculares Avanzados, Instituto de Ecología A.C., Cluster BioMimic®, Carretera Antigua a Coatepec 351, El Haya, Xalapa 91073, Veracruz, Mexico.ORCID 0000-0003-1123-4286
José M Elizalde-ContrerasRed de Estudios Moleculares Avanzados, Instituto de Ecología A.C., Cluster BioMimic®, Carretera Antigua a Coatepec 351, El Haya, Xalapa 91073, Veracruz, Mexico.ORCID 0000-0003-0175-0930
Eliel Ruiz-MayRed de Estudios Moleculares Avanzados, Instituto de Ecología A.C., Cluster BioMimic®, Carretera Antigua a Coatepec 351, El Haya, Xalapa 91073, Veracruz, Mexico.ORCID 0000-0003-1416-4973
Hugo Serrano-PosadaConsejo Nacional de Ciencia y Tecnología, Laboratorio de Biología Sintética, Estructural y Molecular, Laboratorio de Agrobiotecnología, Tecnoparque CLQ, Universidad de Colima, Carretera Los Limones-Loma de Juárez, Colima 28629, Colima, Mexico.ORCID 0000-0002-7901-475X
Juan Alberto Osuna-CastroFacultad de Ciencias Biológicas y Agropecuarias, Universidad de Colima, Carr. Colima-Manzanillo km. 40, Tecomàn 28100, Colima, Mexico.ORCID 0000-0002-4685-5296

Funding

CONACyT grants CB-2016-01-285001, INFR-2017-01-280608, CB-2018-A1-S-18011, CF-2019-33-549477 and INFR-2021-17-316456.
6 · The paper itself

Abstract

Amaranth 11S globulins (Ah11Sn) are an excellent source of essential amino acids; however, there have been no investigations on the characterization of their techno-functional properties at different pH conditions and NaCl concentrations, which are necessary for food formulations. In this work, we report a new two-step purification method for native Ah11Sn with purity levels of ~95%. LC-MS/MS analysis revealed the presence of three different Ah11Sn paralogs named Ah11SB, A11SC, and Ah11SHMW, and their structures were predicted with Alphafold2. We carried out an experimental evaluation of Ah11Sn surface hydrophobicity, solubility, emulsifying properties, and assembly capacity to provide an alternative application of these proteins in food formulations. Ah11Sn showed good surface hydrophobicity, solubility, and emulsifying properties at pH values of 2 and 3. However, the emulsions became unstable at 60 min. The assembly capacity of Ah11Sn evaluated by DLS analysis showed mainly the trimeric assembly (~150-170 kDa). This information is beneficial to exploit and utilize Ah11Sn rationally in food systems.

Indexed as

assembly capacitybioinformatic analysismass spectrometry analysisphysicochemical and functional properties

Identifiers

PMID36765990
PMCPMC9914310

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.