Evidence map›Paper›PMID 36765158›Full record

ArticleISME communications2021

Community RNA-Seq: multi-kingdom responses to living versus decaying roots in soil.

Erin E Nuccio, Nhu H Nguyen, Ulisses Nunes da Rocha, Xavier Mayali, Jeremy Bougoure, Peter K Weber, Eoin Brodie, Mary Firestone, Jennifer Pett-Ridge

Abstract read
In one paragraph

Article in ISME communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. A genomic view of Earth's biomes.Nature reviews. Genetics · 2026
    Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Towards improved biofilm models.Nature reviews. Microbiology · 2025
    Review
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Erin E NuccioPhysical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, USA. nuccio1@llnl.gov.ORCID http://orcid.org/0000-0003-0189-183X
Nhu H NguyenDepartment of Tropical Plant and Soil Sciences, University of Hawai'i at Mānoa, Honolulu, HI, USA.ORCID http://orcid.org/0000-0001-8276-7042
Ulisses Nunes da RochaDepartment of Environmental Microbiology, Helmholtz Centre for Environmental Research, Leipzig, Germany.ORCID http://orcid.org/0000-0001-6972-6692
Xavier MayaliPhysical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, USA.ORCID http://orcid.org/0000-0002-2170-0773
Jeremy BougoureCentre for Microscopy, Characterisation & Analysis, The University of Western Australia, Perth, Australia.
Peter K WeberPhysical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, USA.ORCID http://orcid.org/0000-0001-6022-6050
Eoin BrodieEarth and Environmental Sciences, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.ORCID http://orcid.org/0000-0002-8453-8435
Mary FirestoneEarth and Environmental Sciences, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
Jennifer Pett-RidgePhysical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, USA. pettridge2@llnl.gov.ORCID http://orcid.org/0000-0002-4439-2398

Funding

DOE | SC | Biological and Environmental Research (BER) DE-SC0010570DOE | SC | Biological and Environmental Research (BER) DE-SC0016247DOE | SC | Biological and Environmental Research (BER) DE-SC0020163DOE | SC | Biological and Environmental Research (BER) SCW1421DOE | SC | Biological and Environmental Research (BER) SCW1589DOE | SC | Biological and Environmental Research (BER) SCW1678DOE | SC | Biological and Environmental Research (BER) SCW1711
6 · The paper itself

Abstract

Roots are a primary source of organic carbon input in most soils. The consumption of living and detrital root inputs involves multi-trophic processes and multiple kingdoms of microbial life, but typical microbial ecology studies focus on only one or two major lineages. We used Illumina shotgun RNA sequencing to conduct PCR-independent SSU rRNA community analysis ("community RNA-Seq") and simultaneously assess the bacteria, archaea, fungi, and microfauna surrounding both living and decomposing roots of the annual grass, Avena fatua. Plants were grown in

Identifiers

PMID36765158
PMCPMC9723751

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.