ArticleThe Plant cell2023
The Raf-like MAPKKK INTEGRIN-LINKED KINASE 5 regulates purinergic receptor-mediated innate immunity in Arabidopsis.
Article in The Plant cell, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 37 citations in OpenAlex.
- Defective cuticle-derived signals enhance extracellular ATP response and plant immunity.The New phytologist · 2026Article
- Decoding MAPK cascades in plant immunity: Activation, regulation, integration, and pathogen manipulation.Journal of integrative plant biology · 2026Review
- Rice blast pathogen effector AvrPib compromises disease resistance by targeting Raf-like protein kinase OsMAPKKK72 to inhibit MAPK signaling.Journal of integrative plant biology · 2026Article
- Arabidopsis thaliana Cyclic Nucleotide-Gated Channel 19 is involved in root extracellular ATP and Pep1 signalling.The New phytologist · 2025Article
- DSPicable V: TuMV exploits a dual-specificity phosphatase to suppress MAPK signaling and host resistance.The Plant cell · 2025Article
- Jasmonate primes plant responses to extracellular ATP.The Plant journal : for cell and molecular biology · 2025Article
- Article
- Extracellular ATP: an emerging multifaceted regulator of plant fitness.Plant biotechnology journal · 2025Review
- Identification of mitogen-activated protein kinases substrates inPlant signaling & behavior · 2024Article
- FERONIA orchestrates P2K1-driven purinergic signaling in plant roots.Plant signaling & behavior · 2024Article
- Subfamily C7 Raf-like kinases MRK1, RAF26, and RAF39 regulate immune homeostasis and stomatal opening in Arabidopsis thaliana.The New phytologist · 2024Article
- VIK-Mediated Auxin Signaling Regulates Lateral Root Development in Arabidopsis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- DecodingPlants (Basel, Switzerland) · 2024Article
- CPK28 is a modulator of purinergic signaling in plant growth and defense.The Plant journal : for cell and molecular biology · 2024Article
- ATP homeostasis and signaling in plants.Plant communications · 2024Review
- The Auto-Regulation of ATL2 E3 Ubiquitin Ligase Plays an Important Role in the Immune Response againstInternational journal of molecular sciences · 2024Article
- Unveiling orphan receptor-like kinases in plants: novel client discovery using high-confidence library predictions in the Kinase-Client (KiC) assay.Frontiers in plant science · 2024Article
- Phosphorylation-mediated regulation of integrin-linked kinase 5 by purinoreceptor P2K2.Plant signaling & behavior · 2023Article
- Salt stress releases extracellular ATP to activate purinergic signaling and inhibit plant growth.Plant physiology · 2023Article
- Genome-Wide Identification of thePlants (Basel, Switzerland) · 2023Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Mitogen-activated protein (MAP) kinase signaling cascades play important roles in eukaryotic defense against various pathogens. Activation of the extracellular ATP (eATP) receptor P2K1 triggers MAP kinase 3 and 6 (MPK3/6) phosphorylation, which leads to an elevated plant defense response. However, the mechanism by which P2K1 activates the MAPK cascade is unclear. In this study, we show that in Arabidopsis thaliana, P2K1 phosphorylates the Raf-like MAP kinase kinase kinase (MAPKKK) INTEGRIN-LINKED KINASE 5 (ILK5) on serine 192 in the presence of eATP. The interaction between P2K1 and ILK5 was confirmed both in vitro and in planta and their interaction was enhanced by ATP treatment. Similar to P2K1 expression, ILK5 expression levels were highly induced by treatment with ATP, flg22, Pseudomonas syringae pv. tomato DC3000, and various abiotic stresses. ILK5 interacts with and phosphorylates the MAP kinase MKK5. Moreover, phosphorylation of MPK3/6 was significantly reduced upon ATP treatment in ilk5 mutant plants, relative to wild-type (WT). The ilk5 mutant plants showed higher susceptibility to P. syringae pathogen infection relative to WT plants. Plants expressing only the mutant ILK5S192A protein, with decreased kinase activity, did not activate the MAPK cascade upon ATP addition. These results suggest that eATP activation of P2K1 results in transphosphorylation of the Raf-like MAPKKK ILK5, which subsequently triggers the MAPK cascade, culminating in activation of MPK3/6 associated with an elevated innate immune response.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.