ArticleApplied biosafety : journal of the American Biological Safety Association2022
Autoclaving as a Routine Method for the Decontamination of Animal Carcasses in a Biosafety Level 3 Facility.
Article in Applied biosafety : journal of the American Biological Safety Association, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed, 2 citations in OpenAlex.
- Effect of Common Variables on Autoclave Best Practices in Rodent Barrier Programs.Journal of the American Association for Laboratory Animal Science : JAALAS · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Introduction: Carcasses from animal experiments with RG-3 pathogens should be decontaminated onsite in Austria. Objective: The aim of this study was to find out if the use of pass-through autoclaves for the decontamination of animal carcasses (up to 40 kg of weight) could serve as a routine method for smaller laboratories, as the installation of special carcass decontamination plants may be cost prohibitive. Methods: Biological indicators (BIs) were implanted into the carcasses of animals of different sizes and species with a novel method using stainless steel pipes. The bodies were placed in autoclavable plastic bags and equipped with thermal probes by insertion through the rectum. Subsequently a factory default autoclave cycle for liquids was performed, which holds a core temperature of 121°C for 20 min. Results: The weight of the carcasses ranged from 1 to 42 kg, the duration of the individual cycles reached from 2.2 to 17.23 h. Decontamination was successful every single time as shown by the BIs. The application through the natural orifices with the help of the application tools seems to offer a reliable alternative for implanting the BIs into the carcasses without creating new openings. Insulation properties did not pose substantial challenges to the process. Limitations on the packaging procedure were identified in carcasses larger than 30 kg. Conclusion: Based on the results of this study, using pass-through autoclaves represents an option as a routine method for the decontamination of animal carcasses up to at least 40 kg.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.