ArticleScientific reports2023
Reduced glucosinolate content in oilseed rape (Brassica napus L.) by random mutagenesis of BnMYB28 and BnCYP79F1 genes.
Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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9 citing papers in PubMed, 19 citations in OpenAlex.
- Identification of candidate genes regulating seed oil and protein content in Brassica napus via integrated genome-wide association and transcriptome analysis.BMC plant biology · 2026Article
- Glucosinolates inInternational journal of molecular sciences · 2026Review
- Creating a new oilseed crop, pennycress, by combining key domestication traits using CRISPR genome editing.Nature plants · 2026Article
- In Silico Proteomic Profiling and Bioactive Peptide Potential of Rapeseed Meal.Foods (Basel, Switzerland) · 2025Article
- Is there an oilseed rape ideotype that can efficiently withstand sulfur deficiency?Journal of experimental botany · 2025Article
- Sulforaphane in cancer precision medicine: from biosynthetic origins to multiscale mechanisms and clinical translation.Frontiers in immunology · 2025Review
- Prolonged heat stress inFrontiers in plant science · 2025Article
- Nitrilase GiNIT fromInternational journal of molecular sciences · 2024Article
- Factors Affecting the Quality of Canola Grains and Their Implications for Grain-Based Foods.Foods (Basel, Switzerland) · 2023Review
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Authors and funding
7 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The presence of anti-nutritive compounds like glucosinolates (GSLs) in the rapeseed meal severely restricts its utilization as animal feed. Therefore, reducing the GSL content to < 18 µmol/g dry weight in the seeds is a major breeding target. While candidate genes involved in the biosynthesis of GSLs have been described in rapeseed, comprehensive functional analyses are missing. By knocking out the aliphatic GSL biosynthesis genes BnMYB28 and BnCYP79F1 encoding an R2R3 MYB transcription factor and a cytochrome P450 enzyme, respectively, we aimed to reduce the seed GSL content in rapeseed. After expression analyses on single paralogs, we used an ethyl methanesulfonate (EMS) treated population of the inbred winter rapeseed 'Express617' to detect functional mutations in the two gene families. Our results provide the first functional analysis by knock-out for the two GSL biosynthesis genes in winter rapeseed. We demonstrate that independent knock-out mutants of the two genes possessed significantly reduced seed aliphatic GSLs, primarily progoitrin. Compared to the wildtype Express617 control plants (36.3 µmol/g DW), progoitrin levels were decreased by 55.3% and 32.4% in functional mutants of BnMYB28 (16.20 µmol/g DW) and BnCYP79F1 (24.5 µmol/g DW), respectively. Our study provides a strong basis for breeding rapeseed with improved meal quality in the future.
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