ArticleNature plants2023
Leaf transformation for efficient random integration and targeted genome modification in maize and sorghum.
Article in Nature plants, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
67 citing papers in PubMed, 121 citations in OpenAlex.
- The combination of morphogenic regulators BABY BOOM and GRF-GIF improves maize transformation efficiency and promotes leaf regeneration.The New phytologist · 2026Article
- Boosting genome editing in perennial plants by CRISPR-Combo mediated morphogenic gene activation.Nature communications · 2026Article
- Genome-Wide Characterization of the WOX Gene Family inPlants (Basel, Switzerland) · 2026Article
- Cross-Species Reprogramming of Developmental Plasticity and Metabolic Rewiring via Banana-Derived WUS2 Developmental Regulator.Plant biotechnology journal · 2026Article
- Overexpression ofGenes · 2026Article
- Targeted knockout of a host peroxisomal peptidase confers field resistance to maize lethal necrosis.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Plant Genetic Engineering: Technological Pathways, Application Scenarios, and Future Directions.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Genome-wide identification and expression analysis of the maize ZmWOX gene family reveal its critical role in callus formation.BMC genomics · 2026Article
- Quantifying yield losses from Bt resilience among maize cultivars in South Africa.Nature communications · 2026Article
- Optimisation of Agrobacterium-Mediated Seedling Leaf Transformation Across Multiple Sorghum Genotypes.Plant biotechnology journal · 2026Article
- Mapping cis-regulatory mutations at scale in sorghum enables modulation of gene expression.Nature biotechnology · 2026Article
- Improved efficiency of genetic transformation for the development of elite germplasm using CRISPR/Cas12i3 in maize.aBIOTECH · 2026Article
- KNAT7 transcription factor regulates metabolite and ion profiles to control cell wall biosynthesis in Populus.Scientific reports · 2026Article
- Establishment of an efficientFrontiers in plant science · 2026Article
- How Apomictic and Sexual Cenchrus Species Can Help Elucidate the Mechanisms Leading to Apospory.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Biotechnological and genetic innovations to enhance sorghum adaptation under climate change.Frontiers in plant science · 2026Review
- Transposon-derived genome editors in plants: from compact nucleases to large-fragment integration and regeneration strategies.Engineering in life sciences · 2026Review
- Advances in the use of morphogenic regulators and peptide regenerating factors for boosting plant transformation and genome editing.Frontiers in plant science · 2026Review
- High-throughput genetic transformation and genome editing in pearl millet (Pennisetum glaucum L.).Plant physiology · 2025Article
- Rational design of induced regeneration via somatic embryogenesis in the absence of exogenous phytohormones.The Plant cell · 2025Article
7 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transformation in grass species has traditionally relied on immature embryos and has therefore been limited to a few major Poaceae crops. Other transformation explants, including leaf tissue, have been explored but with low success rates, which is one of the major factors hindering the broad application of genome editing for crop improvement. Recently, leaf transformation using morphogenic genes Wuschel2 (Wus2) and Babyboom (Bbm) has been successfully used for Cas9-mediated mutagenesis, but complex genome editing applications, requiring large numbers of regenerated plants to be screened, remain elusive. Here we demonstrate that enhanced Wus2/Bbm expression substantially improves leaf transformation in maize and sorghum, allowing the recovery of plants with Cas9-mediated gene dropouts and targeted gene insertion. Moreover, using a maize-optimized Wus2/Bbm construct, embryogenic callus and regenerated plantlets were successfully produced in eight species spanning four grass subfamilies, suggesting that this may lead to a universal family-wide method for transformation and genome editing across the Poaceae.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.