ArticlePloS one2023
Theory and practice of using cell strainers to sort Caenorhabditis elegans by size.
Article in PloS one, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Quantifying Embryonic Lethality Rate in Caenorhabditis elegans.Journal of visualized experiments : JoVE · 2026Article
- A Robust Chemical-free Platform for Age-SynchronizedbioRxiv : the preprint server for biology · 2026Article
- Development of a size-separation technique to isolate Caenorhabditis elegans embryos using mesh filters.PloS one · 2025Article
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4 authors.
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Abstract
The nematode Caenorhabditis elegans is a model organism widely used in basic, translational, and industrial research. C. elegans development is characterized by five morphologically distinct stages, including four larval stages and the adult stage. Stages differ in a variety of aspects including size, gene expression, physiology, and behavior. Enrichment for a particular developmental stage is often the first step in experimental design. When many hundreds of worms are required, the standard methods of enrichment are to grow a synchronized population of hatchlings for a fixed time, or to sort a mixed population of worms according to size. Current size-sorting methods have higher throughput than synchronization and avoid its use of harsh chemicals. However, these size-sorting methods currently require expensive instrumentation or custom microfluidic devices, both of which are unavailable to the majority C. elegans laboratories. Accordingly, there is a need for inexpensive, accessible sorting strategies. We investigated the use of low-cost, commercially available cell strainers to filter C. elegans by size. We found that the probability of recovery after filtration as a function of body size for cell strainers of three different mesh sizes is well described by logistic functions. Application of these functions to predict filtration outcomes revealed non-ideal properties of filtration of worms by cell strainers that nevertheless enhanced filtration outcomes. Further, we found that serial filtration using a pair of strainers that have different mesh sizes can be used to enrich for particular larval stages with a purity close to that of synchronization, the most widely used enrichment method. Throughput of the cell strainer method, up to 14,000 worms per minute, greatly exceeds that of other enrichment methods. We conclude that size sorting by cell strainers is a useful addition to the array of existing methods for enrichment of particular developmental stages in C. elegans.
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