Evidence map›Paper›PMID 36757632›Full record

ArticleCurrent protocols2023

Serial Recombineering Cloning to Build Selectable and Tagged Genomic P[acman] BAC Clones for Selection Transgenesis and Functional Gene Analysis using Drosophila melanogaster.

Koen J T Venken, Nick Matinyan, Yezabel Gonzalez, Herman A Dierick

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Current protocols, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.2field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Koen J T VenkenVerna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas.
Nick MatinyanVerna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas.
Yezabel GonzalezVerna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas.
Herman A DierickDepartment of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas.
Baylor College of Medicine · USJohns Hopkins University · US

Funding

TRAINING PROGRAM IN CELL AND MOLECULAR BIOLOGYT32GM008231 · NIGMS · BAYLOR COLLEGE OF MEDICINE · PI NELSON, DAVID LOREN · 1990 to 2019
$7.2M
Peptidergic regulation of aggression in Drosophila melanogasterR01MH107474 · NIMH · BAYLOR COLLEGE OF MEDICINE · PI DIERICK, HERMAN A · 2015 to 2019
$2.0M
The Drosophila Gene Tagging Project: Transgenes with Swappable Protein TagsR21HG006726 · NHGRI · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI HOSKINS, ROGER ALLEN · 2012 to 2013
$486k
Using cell and fly models to understand gene function in undiagnosed diseasesR21GM110190 · NIGMS · BAYLOR COLLEGE OF MEDICINE · PI GRAHAM, BRETT HARRISON · 2014 to 2015
$470k
RESOLVING COMPLEX SYSTEMIC ENDOGENOUS EXPRESSION PATTERNS INTO SUBCELLULAR HIGH-RESOLUTION LOCALIZATIONR21OD022981 · OD · BAYLOR COLLEGE OF MEDICINE · PI VENKEN, KOEN JOZEF THEO · 2016 to 2017
$426k
NHGRI NIH HHS R21 HG006726NIGMS NIH HHS R21 GM110190NIGMS NIH HHS T32 GM008231NIH HHS R01MH107474NIH HHS R21GM110190NIH HHS R21HG006726NIH HHS R21 OD022981NIH HHS R21OD022981NIH HHS T32GM008231NIMH NIH HHS R01 MH107474
6 · The paper itself

Abstract

Transgenes with genomic DNA fragments that encompass genes of interest are the gold standard for complementing null alleles in rescue experiments in the fruit fly Drosophila melanogaster. Of particular interest are genomic DNA clones available as bacterial artificial chromosomes (BACs) or fosmids from publicly available genomic DNA libraries. Genes contained within BAC and fosmid clones can be easily modified by recombineering cloning to insert peptide or protein tags to localize, visualize, or manipulate gene products, and to create point mutations or deletions for structure-function analysis of the inserted genes. However, since transgenesis efficiency is inversely correlated with transgene size, obtaining transgenic animals for increasingly larger BAC and fosmid clones requires increasingly laborious screening efforts using the transgenesis marker commonly used for these transgenes, the dominant eye color marker white

Indexed as

Drosophila melanogasterGene Transfer TechniquesAnimalsAnimals, Genetically ModifiedClone CellsCloning, MolecularDNADrosophilaGenomicsDNAbacterial artificial chromosomeDrosophila melanogastergene expression analysisP[acman]selectionserial recombineering cloningtransgenesis

Identifiers

PMID36757632
PMCPMC9923880
OpenAlexW4319656570

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.