Evidence map›Paper›PMID 36757602›Full record

ArticleCurrent protocols2023

Synthetic Assembly DNA Cloning to Build Plasmids for Multiplexed Transgenic Selection, Counterselection or Any Other Genetic Strategies Using Drosophila melanogaster.

Koen J T Venken, Nick Matinyan, Yezabel Gonzalez, Alejandro Sarrion-Perdigones, Herman A Dierick

Open access · greenAbstract read
In one paragraph

Article in Current protocols, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. GoldenBraid2.0Synthetic biology (Oxford, England) · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Koen J T VenkenVerna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas.
Nick MatinyanVerna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas.
Yezabel GonzalezVerna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas.
Alejandro Sarrion-PerdigonesVerna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas.
Herman A DierickDepartment of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas.
Baylor College of Medicine · USRobert and Janice McNair Foundation · US

Funding

TRAINING PROGRAM IN CELL AND MOLECULAR BIOLOGYT32GM008231 · NIGMS · BAYLOR COLLEGE OF MEDICINE · PI NELSON, DAVID LOREN · 1990 to 2019
$7.2M
Peptidergic regulation of aggression in Drosophila melanogasterR01MH107474 · NIMH · BAYLOR COLLEGE OF MEDICINE · PI DIERICK, HERMAN A · 2015 to 2019
$2.0M
The Drosophila Gene Tagging Project: Transgenes with Swappable Protein TagsR21HG006726 · NHGRI · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI HOSKINS, ROGER ALLEN · 2012 to 2013
$486k
Using cell and fly models to understand gene function in undiagnosed diseasesR21GM110190 · NIGMS · BAYLOR COLLEGE OF MEDICINE · PI GRAHAM, BRETT HARRISON · 2014 to 2015
$470k
RESOLVING COMPLEX SYSTEMIC ENDOGENOUS EXPRESSION PATTERNS INTO SUBCELLULAR HIGH-RESOLUTION LOCALIZATIONR21OD022981 · OD · BAYLOR COLLEGE OF MEDICINE · PI VENKEN, KOEN JOZEF THEO · 2016 to 2017
$426k
NHGRI NIH HHS R21 HG006726NIGMS NIH HHS R21 GM110190NIGMS NIH HHS T32 GM008231NIH HHS R01MH107474NIH HHS R21GM110190NIH HHS R21HG006726NIH HHS R21 OD022981NIH HHS R21OD022981NIH HHS T32GM008231NIMH NIH HHS R01 MH107474
6 · The paper itself

Abstract

We recently described a drug-based selectable and counterselectable genetic platform for the animal model system Drosophila melanogaster, consisting of four resistance and two sensitivity markers that allow direct selection for, or counterselection against, a desired genotype. This platform eliminates the need to identify modified progeny by traditional laborious screening using the dominant eye and body color markers, white

Indexed as

DNADrosophila melanogasterAnimalsAnimals, Genetically ModifiedCloning, MolecularPlasmidsDNAcounterselectionDrosophila melanogastergene expression analysisGoldenBraid 2.0multiplexedselectionsynthetic assembly cloningtransgenesis

Identifiers

PMID36757602
PMCPMC10281009
OpenAlexW4319655555

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.