ArticlePlant cell reports2023
Enhancing alfalfa resistance to Spodoptera herbivory by sequestering microRNA396 expression.
Article in Plant cell reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 26 citations in OpenAlex.
- Identification and characterization of salt-, drought-, and combined salt-drought stress-responsivePlant signaling & behavior · 2026Article
- The multifaceted regulatory network of microRNA396: Orchestrating plant plasticity through coordinating development and various stresses.Journal of integrative plant biology · 2026Review
- Transcriptome and small RNA sequencings reveal the response of tobacco to aphid infestation.BMC genomics · 2025Article
- Genome-wide identification and analysis of miR396 family members and their target GRF genes in rubber tree (Hevea brasiliensis).BMC genomics · 2025Article
- Development and Application of an In Vitro Pollen Viability Assay for Comparative Safety Assessment of Transgenic Alfalfa (Plants (Basel, Switzerland) · 2025Article
- miR825-5p-regulated TNLs govern Arabidopsis resistance to Tetranychus urticae and Pieris brassicae.The New phytologist · 2025Article
- Non-coding RNAs in plant stress responses: molecular insights and agricultural applications.Plant biotechnology journal · 2025Review
- Advances in basic biology of alfalfa (Horticulture research · 2025Article
- Molecular Mechanisms of the Biological Control of Pine Wilt Disease Using Microorganisms.Microorganisms · 2025Review
- Overexpression MsJAR1 gene increased lateral branches and plant height in alfalfa (Medicago sativa L.).Scientific reports · 2025Article
- Blocking miR396 activity by overexpression MIM396 improved switchgrass tiller number and biomass yield.Biotechnology for biofuels and bioproducts · 2024Article
- microRNAs: Key Regulators in Plant Responses to Abiotic and Biotic Stresses via Endogenous and Cross-Kingdom Mechanisms.International journal of molecular sciences · 2024Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
key messageSequestering microRNA396 by overexpression of MIM396 enhanced alfalfa resistance to Spodoptera litura larvae, which may be due to increased lignin content and enhanced low-molecular weight flavonoids and glucosinolates biosynthesis. Alfalfa (Medicago sativa), the most important leguminous forage crop, suffers from the outbreak of defoliator insects, especially Spodoptera litura, resulting in heavy losses in yield and forage quality. Here, we found that the expression of alfalfa microRNA396 (miR396) precursor genes and mature miR396 was significantly up-regulated in wounding treatment that simulates feeding injury by defoliator insects. To verify the function of miR396 in alfalfa resistance to insect, we generated MIM396 transgenic alfalfa plants with significantly down-regulated miR396 expression by Agrobacterium-mediated genetic transformation. The MIM396 transgenic alfalfa plants exhibited improved resistance to Spodoptera litura larvae with increased lignin content but decreased JA accumulation. Most of the miR396 putative target GRF genes were up-regulated in MIM396 transgenic lines, and responded to the wounding treatment. By RNA sequencing analysis, we found that the differentially expressed genes related to insect resistance between WT and MIM396 transgenic plants mainly clustered in biosynthesis pathways in lignin, flavonoids and glucosinolates. In addition to the phenotype of enhanced insect resistance, MIM396 transgenic plants also displayed reduced biomass yield and forage quality. Our results broaden the function of miR396 in alfalfa and provide genetic resources for studying alfalfa insect resistance.
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