Evidence map›Paper›PMID 36755335›Full record

ArticleJournal of cachexia, sarcopenia and muscle2023

tRNA-derived small RNA, 5'tiRNA-Gly-CCC, promotes skeletal muscle regeneration through the inflammatory response.

Linyuan Shen, Tianci Liao, Qiuyang Chen, Yuhang Lei, Linghui Wang, Hao Gu, Yanhao Qiu, Ting Zheng, Yiting Yang, Chenggang Wei and 10 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cachexia, sarcopenia and muscle, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed
6.5field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed, 42 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Review
  5. Article
  6. Article
  7. Article
  8. Review
  9. Article
  10. Review
  11. Article
  12. Article
  13. Article
  14. Review
  15. Review
  16. Review
  17. Article
  18. Article
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors at 3 institutions in 2 countries.

Linyuan ShenCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.
Tianci LiaoCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.
Qiuyang ChenCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.
Yuhang LeiCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.
Linghui WangCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.
Hao GuCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.
Yanhao QiuCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.
Ting ZhengCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.
Yiting YangCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.
Chenggang WeiCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.
Lei ChenCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.
Ye ZhaoCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.
Lili NiuCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.
Shunhua ZhangCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.
Yan ZhuCollege of Life Science, China West Normal University, Nanchong, China.
Mingzhou LiCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.
Jinyong WangChongqing Academy of Animal Science, Chongqing, China.
Xuewei LiCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.
Mailin GanCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.
Li ZhuCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.ORCID 0000-0001-7342-0880
Sichuan Agricultural University · CNChina West Normal University · CNChongqing Academy of Animal Science · CN

Funding

China Agriculture Research System CARS-pig-35National Natural Science Foundation of China 31902135National Natural Science Foundation of China 31972524Sichuan Science and Technology Program 2021YJ0265Sichuan Science and Technology Program 2021ZDZX008Sichuan Science and Technology Program SCSZTD-2023-08-09
6 · The paper itself

Abstract

backgroundIncreasing evidence shows that tRNA-derived small RNAs (tsRNAs) are not only by-products of transfer RNAs, but they participate in numerous cellular metabolic processes. However, the role of tsRNAs in skeletal muscle regeneration remains unknown.

methodsSmall RNA sequencing revealed the relationship between tsRNAs and skeletal muscle injury. The dynamic expression level of 5'tiRNA-Gly after muscle injury was confirmed by real-time quantitative PCR (q-PCR). In addition, q-PCR, flow cytometry, the 5-ethynyl-2'-deoxyuridine (Edu), cell counting kit-8, western blotting and immunofluorescence were used to explore the biological function of 5'tiRNA-Gly. Bioinformatics analysis and dual-luciferase reporter assay were used to further explore the mechanism of action under the biological function of 5'tiRNA-Gly.

resultsTranscriptome analysis revealed that tsRNAs were significantly enriched during inflammatory response immediately after muscle injury. Interestingly, we found that 5'tiRNA-Gly was significantly up-regulated after muscle injury (P < 0.0001) and had a strong positive correlation with inflammation in vivo. In vitro experiments showed that 5'tiRNA-Gly promoted the mRNA expression of proinflammatory cytokines (IL-1β, P = 0.0468; IL-6, P = 0.0369) and the macrophages of M1 markers (TNF-α, P = 0.0102; CD80, P = 0.0056; MCP-1, P = 0.0002). On the contrary, 5'tiRNA-Gly inhibited the mRNA expression of anti-inflammatory cytokines (IL-4, P = 0.0009; IL-10, P = 0.0007; IL-13, P = 0.0008) and the mRNA expression of M2 markers (TGF-β1, P = 0.0016; ARG1, P = 0.0083). Flow cytometry showed that 5'tiRNA-Gly promoted the percentage of CD86

conclusionsThese results reveal that 5'tiRNA-Gly potentially regulated skeletal muscle regeneration by inducing inflammation via the TGF-β signalling pathway. The findings of this study uncover a new potential target for skeletal muscle regeneration treatment.

Indexed as

Muscle, SkeletalRNACytokinesHumansInflammationReceptor, Transforming Growth Factor-beta Type IRegenerationRNA, MessengerRNA, TransferTransforming Growth Factor betaCytokinesReceptor, Transforming Growth Factor-beta Type IRNARNA, MessengerRNA, TransferTransforming Growth Factor betainflammationskeletal muscle regenerationTgfbr1TGF-β signalling pathwaytsRNAs

Identifiers

PMID36755335
PMCPMC10067481
OpenAlexW4319655601

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.