ArticleJournal of molecular evolution2023
Case Studies in the Assessment of Microbial Fitness: Seemingly Subtle Changes Can Have Major Effects on Phenotypic Outcomes.
Article in Journal of molecular evolution, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Environment-dependent epistasis shapes adaptation to fluctuating resources.bioRxiv : the preprint server for biology · 2026Article
- Additive effects of environmental and demographic variation shape the repeatability of evolution across replicated experiments.Evolution letters · 2026Article
- Article
- Genome-wide analysis exploring mechanisms used bymSystems · 2026Article
- Evolutionary constraints on RNA polymerase gene positioning in the genome of fast-growing bacteria.Nucleic acids research · 2026Article
- Historical contingency limits adaptive diversification in a spatially structured environment.Evolution letters · 2026Article
- Fitness costs and persistence of plasmid-mediated cephalosporin resistance inFrontiers in microbiology · 2026Review
- Asymmetric interactions and feast-famine cycles drive chaos in microbial populations.Research square · 2025Article
- Site-selective modifications by lipid A phosphoethanolamine transferases linked to colistin resistance and bacterial fitness.mSphere · 2024Article
- Nutrient-rich spatial refuges buffer against extinction and promote evolutionary rescue in evolving microbial populations.Proceedings. Biological sciences · 2024Article
- Single-cell heterogeneity in ribosome content and the consequences for the growth laws.bioRxiv : the preprint server for biology · 2024Article
- Evolution of pH-sensitive transcription termination inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- In Vitro Resistance-Predicting Studies and In Vitro Resistance-Related Parameters-A Hit-to-Lead Perspective.Pharmaceuticals (Basel, Switzerland) · 2024Review
- Trade-offs, trade-ups, and high mutational parallelism underlie microbial adaptation during extreme cycles of feast and famine.Current biology : CB · 2024Article
- Evolution of pH-sensitive transcription termination during adaptation to repeated long-term starvation.bioRxiv : the preprint server for biology · 2024Article
- Identifying Targets of Selection in Laboratory Evolution Experiments.Journal of molecular evolution · 2023Review
- Extreme Sensitivity of Fitness to Environmental Conditions: Lessons from #1BigBatch.Journal of molecular evolution · 2023Article
- Best Practices in Microbial Experimental Evolution.Journal of molecular evolution · 2023Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Following the completion of an adaptive evolution experiment, fitness evaluations are routinely conducted to assess the magnitude of adaptation. In doing so, proper consideration should be given when determining the appropriate methods as trade-offs may exist between accuracy and throughput. Here, we present three instances in which small changes in the framework or execution of fitness evaluations significantly impacted the outcomes. The first case illustrates that discrepancies in fitness conclusions can arise depending on the approach to evaluating fitness, the culture vessel used, and the sampling method. The second case reveals that variations in environmental conditions can occur associated with culture vessel material. Specifically, these subtle changes can greatly affect microbial physiology leading to changes in the culture pH and distorting fitness measurements. Finally, the last case reports that heterogeneity in CFU formation time can result in inaccurate fitness conclusions. Based on each case, considerations and recommendations are presented for future adaptive evolution experiments.
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