Evidence map›Paper›PMID 36750863›Full record

ArticleActa neuropathologica communications2023

Identification and gene expression profiling of human gonadotrophic pituitary adenoma stem cells.

Linhao Yuan, Peiliang Li, Jiang Li, Jiayi Peng, Jianlong Zhouwen, Shunchang Ma, Guijun Jia, Wang Jia, Peng Kang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta neuropathologica communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.8field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Distribution, contribution and regulation of nestinJournal of advanced research · 2024
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 1 country.

Linhao YuanDepartment of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.
Peiliang LiDepartment of Neurosurgery, Beijing Ditan Hospital, Capital Medical University, Chaoyang District, Beijing, China.
Jiang LiDepartment of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.
Jiayi PengDepartment of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.
Jianlong ZhouwenDepartment of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.
Shunchang MaChina National Clinical Research Center for Neurological Diseases, Fengtai, Beijing, China.
Guijun JiaDepartment of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.
Wang JiaDepartment of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China. jwttyy@126.com.
Peng KangDepartment of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China. kangpeng@bjtth.org.
Capital Medical University · CNNational Clinical Research Center for Digestive Diseases · CNBeijing Institute of Neurosurgery · CNBeijing Tian Tan Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundGonadotrophic pituitary adenoma is a major subtype of pituitary adenoma in the sellar region, but it is rarely involved in the hypersecretion of hormones into blood; thus, it is commonly regarded as "non-functioning." Its tumorigenic mechanisms remain unknown. The aim of this study was to identify human gonadotrophic pituitary adenoma stem cells (hPASCs) and explore the underlying gene expression profiles. In addition, the potential candidate genes involved in the invasive properties of pituitary adenoma were examined.

methodsThe hPASCs from 14 human gonadotrophic pituitary adenoma clinical samples were cultured and verified via immunohistochemistry. Genetic profiling of hPASCs and the matched tumor cells was performed through RNA-sequencing and subjected to enrichment analysis. By aligning the results with public databases, the candidate genes were screened and examined in invasive and non-invasive gonadotrophic pituitary adenomas using Real-time polymerase chain reaction.

resultsThe hPASCs were successfully isolated and cultured from gonadotrophic pituitary adenoma in vitro, which were identified as positive for generic stem cell markers (Sox2, Oct4, Nestin and CD133) via immunohistochemical staining. The hPASCs could differentiate into the tumor cells expressing follicle-stimulating hormone in the presence of fetal bovine serum in the culture medium. Through RNA-sequencing, 1352 differentially expressed genes were screened and identified significantly enriched in various gene ontologies and important pathways. The expression levels of ANXA2, PMAIP1, SPRY2, C2CD4A, APOD, FGF14 and FKBP10 were significantly upregulated while FNDC5 and MAP3K4 were downregulated in the invasive gonadotrophic pituitary adenomas compared to the non-invasive ones.

conclusionGenetic profiling of hPASCs may explain the tumorigenesis and invasiveness of gonadotrophic pituitary adenoma. ANXA2 may serve as a potential therapeutic target for the treatment of gonadotrophic pituitary adenoma.

Indexed as

AdenomaPituitary NeoplasmsFibronectinsGene Expression ProfilingHumansIntracellular Signaling Peptides and ProteinsMembrane ProteinsMicroarray AnalysisRNAStem CellsFibronectinsFNDC5 protein, humanIntracellular Signaling Peptides and ProteinsMembrane ProteinsRNASPRY2 protein, humanGonadotrophic pituitary adenomaRNA-sequencingTumor stem cells

Identifiers

PMID36750863
PMCPMC9906881
OpenAlexW4319349454

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.