Evidence map›Paper›PMID 36749534›Full record

ArticleVeterinary research communications2023

Techno-functional characterization of fecal lactobacilli isolates of Bos indicus calves for probiotic properties.

Rakesh Chouraddi, Sachin Kumar, Brijesh Kumar, Manika Bhatia, Vinay Venkatesh Varada, Nitin Tyagi, Rashmi Hogarehalli Mallapa

Abstract read
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In one paragraph

Article in Veterinary research communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
2.6field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Screening of lactic acid bacteria from the milk of Sahiwal cows and characterization of their probiotic potential for preventing bovine mastitis.International microbiology : the official journal of the Spanish Society for Microbiology · 2025
    Article
  5. Probiotic Characteristics and Anti-Inflammatory Effects ofAntioxidants (Basel, Switzerland) · 2024
    Article
  6. Article
  7. Article
  8. UnderstandingFoods (Basel, Switzerland) · 2024
    Review
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Rakesh Chouraddi *Rumen Biotechnology Lab, Animal Nutrition Division, ICAR-National Dairy Research Institute, Karnal, 132001, Haryana, India.
Sachin Kumar *Rumen Biotechnology Lab, Animal Nutrition Division, ICAR-National Dairy Research Institute, Karnal, 132001, Haryana, India. arensachin@gmail.com.
Brijesh KumarRumen Biotechnology Lab, Animal Nutrition Division, ICAR-National Dairy Research Institute, Karnal, 132001, Haryana, India.
Manika BhatiaRumen Biotechnology Lab, Animal Nutrition Division, ICAR-National Dairy Research Institute, Karnal, 132001, Haryana, India.
Vinay Venkatesh VaradaRumen Biotechnology Lab, Animal Nutrition Division, ICAR-National Dairy Research Institute, Karnal, 132001, Haryana, India.
Nitin TyagiRumen Biotechnology Lab, Animal Nutrition Division, ICAR-National Dairy Research Institute, Karnal, 132001, Haryana, India.
Rashmi Hogarehalli MallapaDairy Microbiology Division, ICAR-National Dairy Research Institute, Karnal, 132001, Haryana, India.
National Dairy Research Institute · IN

Funding

ICAR-NCVTCC, Hisar, India NCVTC/Budget/2019-20/860
6 · The paper itself

Abstract

In this study, 105 bacterial colonies were isolated from the feces of newborn healthy Bos indicus calves and 37 isolates were confirmed using morphological, biochemical tests, and genus-specific PCR as lactobacilli. 11 isolates were then short-listed for in vitro probiotic testing based on their ability to dwell under acid and bile stress. Species-level identification using 16S rRNA gene sequencing revealed that they were Ligilactobacillus salivarius. These isolates flourished in 0.4% phenol, depicting resistance in adverse conditions encountered in the gastrointestinal tract. The results of cell surface hydrophobicity were found to be 74.50% for RBL12 and 62.62% for RBL09 in hexadecane and xylene, respectively, and that of auto-aggregation was highest in RBL26 (58.92%). These isolates also produced digestive enzymes like amylase, protease, and β-galactosidase. Further assays reiterated their antimicrobial and coaggregation potential against diarrhea-causing pathogens like Escherichia coli ATCC-25922 and Salmonella arizonae ATCC-13314. Biosafety assessment revealed that none of the tested isolates were hemolytic and mucinolytic in nature. Furthermore, the antioxidant potential of the isolates was also confirmed using 1,1‑diphenyl‑2‑picrylhydrazyl (DPPH) and ferric ion-reducing antioxidant power (FRAP) assay. Along with efficient utilization of inulin, isolates showed promising adhesion ability to the HT-29 cell line. The current findings hence conclude that these Lactobacillus isolates can be exploited as animal probiotics for potential application in young calves to foster gut health and immunity.

Indexed as

LactobacillusProbioticsAnimalsAnti-Bacterial AgentsAntioxidantsCattleFecesRNA, Ribosomal, 16SAnti-Bacterial AgentsAntioxidantsRNA, Ribosomal, 16SBos indicusCell adherenceLactobacillusPrebiotic utilization efficiencyProbiotic

Identifiers

PMID36749534
OpenAlexW4319333166

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.