Evidence map›Paper›PMID 36747833›Full record

ArticlebioRxiv : the preprint server for biology2023

Bacterial mechanosensing of surface stiffness promotes signaling and growth leading to biofilm formation by

Liyun Wang, Yu-Chern Wong, Joshua M Correira, Megan Wancura, Chris J Geiger, Shanice S Webster, Benjamin J Butler, George A O'Toole, Richard M Langford, Katherine A Brown and 4 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 5 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

14 authors at 4 institutions in 4 countries.

Liyun WangDepartment of Physics, Center for Nonlinear Dynamics, The University of Texas at Austin, Austin, TX 78712, USA.
Yu-Chern WongDepartment of Physics, Center for Nonlinear Dynamics, The University of Texas at Austin, Austin, TX 78712, USA.
Joshua M CorreiraDepartment of Chemistry, The University of Texas at Austin, Austin, TX 78712 USA.
Megan WancuraDepartment of Chemistry, The University of Texas at Austin, Austin, TX 78712 USA.
Chris J GeigerGeisel School of Medicine at Dartmouth, Hanover, NH 03755 USA.
Shanice S WebsterGeisel School of Medicine at Dartmouth, Hanover, NH 03755 USA.
Benjamin J ButlerSurfaces, Microstructure and Fracture Group, Cavendish Laboratory, University of Cambridge, Cambridge CB3 0HE, United Kingdom.
George A O'TooleGeisel School of Medicine at Dartmouth, Hanover, NH 03755 USA.
Richard M LangfordSurfaces, Microstructure and Fracture Group, Cavendish Laboratory, University of Cambridge, Cambridge CB3 0HE, United Kingdom.
Katherine A BrownSurfaces, Microstructure and Fracture Group, Cavendish Laboratory, University of Cambridge, Cambridge CB3 0HE, United Kingdom.
Berkin DortdivanliogluDepartment of Civil, Architectural, and Environmental Engineering, The University of Texas at Austin, Austin, TX 78712 USA.
Lauren WebbDepartment of Chemistry, The University of Texas at Austin, Austin, TX 78712 USA.
Elizabeth Cosgriff-HernandezDepartment of Biomedical Engineering, The University of Texas at Austin, Austin, TX 78712 USA.
Vernita D GordonDepartment of Physics, Center for Nonlinear Dynamics, The University of Texas at Austin, Austin, TX 78712, USA.
The University of Texas at Austin · USDartmouth College · USUniversity of Cambridge · GBMax Planck Institute for Terrestrial Microbiology · DE

Funding

cdiGMP regulation of Biofilm FormationR37AI083256 · NIAID · DARTMOUTH COLLEGE · PI O'TOOLE, GEORGE A. · 2014 to 2023
$4.7M
Assessing the roles of biofilm structure and mechanics in pathogenic, persistent infectionsR01AI121500 · NIAID · UNIVERSITY OF TEXAS AT AUSTIN · PI GORDON, VERNITA DIANE · 2017 to 2020
$1.5M
NIAID NIH HHS R01 AI121500NIAID NIH HHS R37 AI083256
6 · The paper itself

Abstract

The attachment of bacteria onto a surface, consequent signaling, and the accumulation and growth of the surface-bound bacterial population are key initial steps in the formation of pathogenic biofilms. While recent reports have hinted that the stiffness of a surface may affect the accumulation of bacteria on that surface, the processes that underlie bacterial perception of and response to surface stiffness are unknown. Furthermore, whether, and how, the surface stiffness impacts biofilm development, after initial accumulation, is not known. We use thin and thick hydrogels to create stiff and soft composite materials, respectively, with the same surface chemistry. Using quantitative microscopy, we find that the accumulation, motility, and growth of the opportunistic human pathogen

Indexed as

bacterial mechanosensingbiofilmbiomechanicscell envelope mechanicscyclic-di-GMPPilTPilY1Pseudomonas aeruginosasurface stiffness sensing

Identifiers

PMID36747833
PMCPMC9900894
OpenAlexW4318275365

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.