Evidence map›Paper›PMID 36747778›Full record

ArticlebioRxiv : the preprint server for biology2023

Time resolution in cryo-EM using a novel PDMS-based microfluidic chip assembly and its application to the study of HflX-mediated ribosome recycling.

Sayan Bhattacharjee, Xiangsong Feng, Suvrajit Maji, Prikshat Dadhwal, Zhening Zhang, Zuben P Brown, Joachim Frank

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors.

Sayan BhattacharjeeDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10027, USA.
Xiangsong FengDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10027, USA.
Suvrajit MajiDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10027, USA.
Prikshat DadhwalDepartment of Biological Sciences, Columbia University, New York, NY 10027, USA.
Zhening ZhangDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10027, USA.
Zuben P BrownDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10027, USA.
Joachim FrankDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10027, USA.

Funding

Structural Studies of Macromolecular Assemblies Using Cryo-EMR35GM139453 · NIGMS · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI FRANK, JOACHIM · 2021 to 2025
$3.9M
NIGMS NIH HHS R35 GM139453
6 · The paper itself

Abstract

The rapid kinetics of biological processes and associated short-lived conformational changes pose a significant challenge in attempts to structurally visualize biomolecules during a reaction in real time. Conventionally, on-pathway intermediates have been trapped using chemical modifications or reduced temperature, giving limited insights. Here we introduce a novel time-resolved cryo-EM method using a reusable PDMS-based microfluidic chip assembly with high reactant mixing efficiency. Coating of PDMS walls with SiO

Identifiers

PMID36747778
PMCPMC9900803

What OpenQuestion holds

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LicenceCC BY-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.