Evidence map›Paper›PMID 36747528›Full record

ArticleHeliyon2023

Transplantation of hybrid adipose-derived stem cell sheet with autologous peritoneum: An

Natsuki Matsuo, Takeshi Ohki, Shota Aoyama, Shigeki Yamaguchi, Michio Itabashi, Hiroto Egawa, Masakazu Yamamoto

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Heliyon, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
2.0field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Natsuki MatsuoDepartment of Surgery, Institute of Gastroenterology, Tokyo Women's Medical University, 8-1 Kawada-Cho Shinjuku-ku Tokyo, 162-8666, Japan.
Takeshi OhkiDepartment of Surgery, Institute of Gastroenterology, Tokyo Women's Medical University, 8-1 Kawada-Cho Shinjuku-ku Tokyo, 162-8666, Japan.
Shota AoyamaDepartment of Surgery, Institute of Gastroenterology, Tokyo Women's Medical University, 8-1 Kawada-Cho Shinjuku-ku Tokyo, 162-8666, Japan.
Shigeki YamaguchiDepartment of Surgery, Institute of Gastroenterology, Tokyo Women's Medical University, 8-1 Kawada-Cho Shinjuku-ku Tokyo, 162-8666, Japan.
Michio ItabashiDepartment of Surgery, Institute of Gastroenterology, Tokyo Women's Medical University, 8-1 Kawada-Cho Shinjuku-ku Tokyo, 162-8666, Japan.
Hiroto EgawaDepartment of Surgery, Institute of Gastroenterology, Tokyo Women's Medical University, 8-1 Kawada-Cho Shinjuku-ku Tokyo, 162-8666, Japan.
Masakazu YamamotoDepartment of Gastroenterological Surgery, Utsunomiya Memorial Hospital, 1-3-16 Ohdori Utsunomiya-shi Tochigi, 320-0811, Japan.
Tokyo Women's Medical University · JPSaiseikai Utsunomiya hospital · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: In regenerative medicine, cell sheet engineering has various advantages, including the retention of cells at the transplantation site for a longer period and the local delivery of growth factors and cytokines. Adipose-derived stem cell (ASC) is widely used owing to their various functions such as wound healing, immunomodulation, and nerve regeneration, in addition to their ability to differentiate into adipocytes, chondrocytes, and osteoblasts. ASC sheet generated using cell sheet engineering is considered effective in preventing anastomotic leakage, a serious postoperative complication in gastrointestinal surgery. However, the ASC sheet is too soft, thin, and brittle to handle with laparoscopic forceps during the operation. Therefore, we considered using the peritoneum, which is stiff and easy to collect while operating, as an alternative support. In this study, we explored the feasibility of using the peritoneum as a support for the precise transplantation of ASC sheets during surgery. Methods: ASCs were isolated from the subcutaneous fat of the inguinal region of Sprague-Dawley (SD) transgenic rats expressing green fluorescent protein. ASCs were cultured until passage 3, seeded in temperature-responsive culture dishes, and the resulting ASC sheet was harvested at more than 80% confluency. Non-transgenic SD rats were used for transplant experiments. The wall peritoneum was harvested from SD rats following laparotomy, and hybrid adipose-derived stem cell (HASC) sheet was prepared by laminating the peritoneum with ASC sheet. The cell sheets were transplanted on the backs of SD rats following the incision. On post-transplantation days 3 and 7, the specimens were extracted. ASC and HASC sheets were then compared macroscopically and histopathologically. Results: HASC sheet transplantation was macroscopically and histopathologically more effective than ASC sheet transplantation. The peritoneum provided sufficient stiffness as a support for precise transplantation. Conclusion: The newly developed HASC sheet, which combine the advantages of ASC sheet with those of the peritoneum, could be more useful for clinical application than the ASC sheet alone.

Indexed as

Adipose-derived stem cell sheetAnastomotic leakageASC, Adipose-derived stem cellCell sheet engineeringHASC, Hybrid adipose-derived stem cellHybrid adipose-derived stem cell sheetMSC, Mesenchymal stem cellPeritoneumPIPAAm, Poly N-isopropylacrylamideSD rats, Sprague Dawley ratsSD-Tg rats, Sprague Dawley transgenic ratsTCPS, Tissue culture polystyrene surfaceTemperature-responsive culture dishes

Identifiers

PMID36747528
PMCPMC9898601
OpenAlexW4316171036

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.