ArticleFrontiers in cellular neuroscience2022
Oligodendrogenesis and myelination tracing in a CRISPR/Cas9-engineered brain microphysiological system.
Article in Frontiers in cellular neuroscience, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 31 citations in OpenAlex.
- CRISPR and Myelin regeneration: a systematic review of applications in demyelinating CNS Disorders, with a focus on MS.Regenerative medicine · 2025Pooled it
- Cryopreservation of brain organoids - a tool for on-demand organoid banking.bioRxiv : the preprint server for biology · 2026Article
- Effects of Sevoflurane on the Development of a Human Brain Microphysiological System.International journal of molecular sciences · 2026Article
- Preconfigured neuronal firing sequences in human brain organoids.Nature neuroscience · 2026Article
- From reference epigenomes to response epigenomes: a public-health case built on the developing brain.Frontiers in public health · 2026Article
- Review
- Multi-Region Brain Organoids Integrating Cerebral, Mid-Hindbrain, and Endothelial Systems.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Human neural organoid microphysiological systems show the building blocks necessary for basic learning and memory.Communications biology · 2025Article
- 2024 International Academy of Toxicologic Pathology (IATP) Satellite Symposium: New Approach Methodologies (NAMs) for Neurotoxicity Assessment and Regulatory Perspectives.Toxicologic pathology · 2025Article
- Microglia-containing neural organoids as brain microphysiological systems for long-term culture.Frontiers in cellular neuroscience · 2025Article
- Advancing next-generation brain organoid platforms for investigating traumatic brain injury from repeated blast exposures.Frontiers in bioengineering and biotechnology · 2025Review
- A glia-enriched stem cell 3D model of the human brain mimics the glial-immune neurodegenerative phenotypes of multiple sclerosis.Cell reports. Medicine · 2024Article
- A Novel Approach to Increase Glial Cell Populations in Brain Microphysiological Systems.Advanced biology · 2024Article
- Stem cell engineering approaches for investigating glial cues in central nervous system disorders.Current opinion in biotechnology · 2024Review
- Review
- Blood extracellular vesicles carrying brain-specific mRNAs are potential biomarkers for detecting gene expression changes in the female brain.Molecular psychiatry · 2024Article
- Article
- Exploring the therapeutic potential of natural compounds modulating the endocannabinoid system in various diseases and disorders: review.Pharmacological reports : PR · 2023Review
- Human brain microphysiological systems in the study of neuroinfectious disorders.Experimental neurology · 2023Review
- First Organoid Intelligence (OI) workshop to form an OI community.Frontiers in artificial intelligence · 2023Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Introduction: Oligodendrocytes (OLs) are the myelin-forming cells of the central nervous system (CNS). Although OLs can be differentiated from human-induced pluripotent stem cells (hiPSCs), the Methods: Here, we take advantage of CRISPR/Cas9 technology to generate a hiPSC line in which proteolipid protein 1 (PLP1), an OLs marker, is tagged with super-fold GFP (sfGFP). While generating the PLP1-sfGFP reporter, we used reverse transfection and obtained higher Knock-In (KI) efficiency compared to forward transfection (61-72 vs. 46%). Results: After validation of the KI and quality control of the PLP1-sfGFP line, selected clones were differentiated into bMPS, and the fidelity, specificity, and function of the tagged PLP protein were verified in this model. We tracked different stages of oligodendrogenesis in the verified lines based on PLP1-sfGFP Discussion: This work demonstrates an efficient method for generating hiPSC KI lines and the description of a new 3D model to study OL differentiation, migration, and maturation both during
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.