ArticleFrontiers in cellular neuroscience2023
Microglia isolation from aging mice for cell culture: A beginner's guide.
Article in Frontiers in cellular neuroscience, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Immune checkpoint LAG-3 governs stage-dependent and disease-associated microglial modules in ALS model mice.Journal of neuroinflammation · 2026Article
- Microglial HVCN1 Deficiency Improves Movement and Survival of SOD1Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Inflammatory stimulus enhances synaptic material uptake by adult APP microglia in a microfluidic neuron-microglia co-culture model.Journal of neuroinflammation · 2026Article
- Microglial CX3CR1 deficiency regulates the selective vulnerability of cone photoreceptors via STAT3/CCL-ACKR1 signaling in the mouse retina.Experimental & molecular medicine · 2026Article
- A validated method for banking mixed glial cultures that yield responsive microglia.bioRxiv : the preprint server for biology · 2025Article
- Temporal biphasic regulation of photoreceptor degeneration by microglial TREM2: A metabolic-immune nexus in retinitis pigmentosa.Science advances · 2025Article
- Novel In Vitro Culture of Microglia from Aged Mice: Implications for the Future of Aging Neurobiology Research.Current protocols · 2025Article
- Dissection of Neurochemical Pathways Across Complexity and Scale.Journal of neurochemistry · 2025Review
- In vitro models of microglia: a comparative study.Scientific reports · 2025Article
- Huang-Lian-Jie-Du decoction alleviates cognitive deficits in Alzheimer's disease model 5xFAD mice by inhibiting Trem2/Dap12 signaling pathway.Chinese medicine · 2025Article
- Article
- Isolation of Primary Brain Cells: Challenges and Solutions.Archives of clinical and biomedical research · 2025Article
- The role of cellular senescence in neurodegenerative diseases.Archives of toxicology · 2024Review
- Impact of microglia isolation and culture methodology on transcriptional profile and function.Journal of neuroinflammation · 2024Article
- Current methods for the microglia isolation: Overview and comparative analysis of approaches.Cell and tissue research · 2024Review
- Reconstitution of human microglia and resident T cells in the brain of humanized DRAGA mice.Frontiers in cellular and infection microbiology · 2024Article
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5 authors.
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Abstract
Microglia, the innate immune cell of the central nervous system, play significant roles in brain development, maintenance, homeostasis, and neuroinflammation. Although numerous methods have been developed to isolate microglia from embryonic or postnatal mouse brains, still major difficulties exist in isolating microglia from adult mice, often resulting in low yield and risk of cellular activation. Therefore, there is a need for a more efficient method to isolate pure and high-yield microglia from adult mice to study various neurodegenerative diseases. The aim of this study was to develop a fully functional protocol for the isolation of microglia by comparing different protocols. We investigated the efficacy of three protocols in terms of cell yield, purity, cellular activation, cellular aging, and migration properties and proposed the modified protocol (PROTOCOL 1), which provides an optimal yield of functional microglial cells with a minimum of material and equipment and allows young researchers with little experience to isolate microglia and helps them to delve deeper into the world of neuroscience.
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