ArticleSensors and actuators. B, Chemical2023
RT-RPA-Cas12a-based assay facilitates the discrimination of SARS-CoV-2 variants of concern.
Article in Sensors and actuators. B, Chemical, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed.
- Rapid visual detection of Treponema pallidum using the RPA-CRISPR/Cas12a system.Scientific reports · 2026Article
- Development of a Rapid Visual Detection Assay for Duck Tembusu Virus Using RT-LAMP-CRISPR/Cas12a.Animals : an open access journal from MDPI · 2024Article
- Simple SARS-CoV-2 concentration methods for wastewater surveillance in low resource settings.The Science of the total environment · 2024Article
- Article
- Interference reduction isothermal nucleic acid amplification strategy for COVID-19 variant detection.Sensors and actuators. B, Chemical · 2023Article
Corrections and comments
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Authors and funding
17 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Timely and accurate detection of SARS-CoV-2 variants of concern (VOCs) is urgently needed for pandemic surveillance and control. Great efforts have been made from a mass of scientists in increasing the detection sensitivity and operability, and reducing the turn-around time and cost. Here, we report a nucleic acid testing-based method aiming to detect and discriminate SARS-CoV-2 mutations by combining RT-RPA and CRISPR-Cas12a detecting assays (RRCd). With a detection limit of 10 copies RNA/reaction, RRCd was validated in 194 clinical samples, showing 89% positive predictive agreement and 100% negative predictive agreement, respectively. Critically, using specific crRNAs, representatives of single nucleotide polymorphisms and small deletions in SARS-CoV-2 VOCs including N501Y, T478K and ΔH69-V70 were discriminated by RRCd, demonstrating 100% specificity in clinical samples with
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