ArticleFrontiers in genome editing2023
On-site and visual detection of sorghum mosaic virus and rice stripe mosaic virus based on reverse transcription-recombinase-aided amplification and CRISPR/Cas12a.
Article in Frontiers in genome editing, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 14 citations in OpenAlex.
- In Situ Visual Detection of TelMV, EAPV, and PaMoV in Passionfruit Using Reverse Transcription-Recombinase-Aided Amplification and CRISPR/Cas12a.Plants (Basel, Switzerland) · 2026Article
- CRISPR-Cas systems for plant virus management: detection, surveillance, and host resistance.Frontiers in plant science · 2026Review
- Plant negative-strand RNA virus phosphoprotein condensates exploit host trafficking and lipid synthesis for viral factory assembly.Science advances · 2025Article
- CRISPR-Cas-Based Diagnosis of Geminiviruses.Methods in molecular biology (Clifton, N.J.) · 2025Article
- A novel system with robust compatibility and stability for detectingMicrobiology spectrum · 2024Article
- Development of a CRISPR/SHERLOCK-Based Method for Rapid and Sensitive Detection of Selected Pospiviroids.Viruses · 2024Article
- Isothermal Nucleic Acid Amplification-Based Lateral Flow Testing for the Detection of Plant Viruses.International journal of molecular sciences · 2024Review
- Rhabdovirus encoded glycoprotein induces and harnesses host antiviral autophagy for maintaining its compatible infection.Autophagy · 2024Article
- Inoculation and Screening Methods for Major Sorghum Diseases Caused by Fungal Pathogens:Plants (Basel, Switzerland) · 2023Review
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rapid, sensitive and visual detection of plant viruses is conducive to effective prevention and control of plant viral diseases. Therefore, combined with reverse transcription and recombinase-aided amplification, we developed a CRISPR/Cas12a-based visual nucleic acid detection system targeting sorghum mosaic virus and rice stripe mosaic virus, which cause harm to crop production in field. When the RT-RAA products were recognized by crRNA and formed a complex with LbCas12a, the ssDNA labeled with a quenched green fluorescent molecule will be cleaved by LbCas12a, and then a significant green fluorescence signal will appear. The entire detection process can be completed within 30 min without using any sophisticated equipment and instruments. The detection system could detect samples at a dilution of 10
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