ArticleFrontiers in microbiology2022
Real-time polymerase chain reaction methods for strain specific identification and enumeration of strain
Article in Frontiers in microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 17 citations in OpenAlex.
- Rapid On-Site Detection ofFoods (Basel, Switzerland) · 2026Article
- Revisiting Strategies for Bacterial Enumeration: The Case for Viable but Nonculturable (VBNC) Cells in Supplement Products.Probiotics and antimicrobial proteins · 2026Review
- Comprehensive in vitro probiotic screening and ITS-based molecular identification and characterization of yeast strains isolated from agro-industrial and rumen sources.BMC microbiology · 2026Article
- Evaluating the Quality of Selected Commercial Probiotic Products, Both Dietary Supplements and Foods for Special Medical Purposes.Foods (Basel, Switzerland) · 2026Article
- Thermal Versus Ultrasound Inactivation of Bifidobacterium animalis subsp. lactis BB-12: Functional Implications for Postbiotics.Probiotics and antimicrobial proteins · 2026Article
- A portable device for probiotic counting and viability assessment based on microfluidic chip and image recognition.iMetaOmics · 2025Article
- Postbiotics: a perspective on their quantification.Frontiers in nutrition · 2025Article
- Real-time PCR methods for identification and stability monitoring ofFrontiers in microbiology · 2024Article
- Viability-PCR for the selective detection ofFrontiers in microbiology · 2024Article
- Development and validation of a PMA-qPCR method for accurate quantification of viableFrontiers in microbiology · 2024Article
- Assessing probiotic viability in mixed species yogurt using a novel propidium monoazide (PMAxx)-quantitative PCR method.Frontiers in microbiology · 2024Article
- Review
- The power of DNA based methods in probiotic authentication.Frontiers in microbiology · 2023Article
- Probiotic and postbiotic analytical methods: a perspective of available enumeration techniques.Frontiers in microbiology · 2023Review
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Introduction: Reliable and accurate methods for probiotic identification and enumeration, at the strain level plays a major role in confirming product efficacy since probiotic health benefits are strain-specific and dose-dependent. In this study, real-time PCR methods were developed for strain specific identification and enumeration of Methods: The assay was designed to target a unique region in Results: The identification method proved to be strain specific and highly sensitive with a limit of detection of 0.5 pg of DNA. The optimal viability dye (PMAxx) concentration was 50 μM. The method was efficient (> 90% with Discussion: The difference between PC and v-PCR methods can potentially be attributed to the proportion of cells that exist in a viable but non culturable (VBNC) state, which can be count by v-PCR but not with PC. The developed v-qPCR method was confirmed to be strain specific, sensitive, efficient, with low variance, able to count VBNC cells, and has shorter time to results compared to plate count methods. Thus, the identification and enumeration methods developed for
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