Evidence map›Paper›PMID 36737435›Full record

ArticleNature communications2023

Shark nanobodies with potent SARS-CoV-2 neutralizing activity and broad sarbecovirus reactivity.

Wei-Hung Chen, Agnes Hajduczki, Elizabeth J Martinez, Hongjun Bai, Hanover Matz, Thomas M Hill, Eric Lewitus, William C Chang, Layla Dawit, Caroline E Peterson and 35 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
6.6field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 34 citations in OpenAlex.

  1. Article
  2. Nanobodies targeting SARS-CoV-2 variants.Acta pharmaceutica Sinica. B · 2026
    Review
  3. Review
  4. Article
  5. Article
  6. Review
  7. Article
  8. Article
  9. The unexpected role of nurse shark pancreas as a secondary lymphoid organ.Journal of immunology (Baltimore, Md. : 1950) · 2025
    Article
  10. Article
  11. Article
  12. Article
  13. Review
  14. Review
  15. Review
  16. Characteristics and Genomic Localization of Nurse Shark (International journal of molecular sciences · 2024
    Article
  17. Review
  18. Article
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

45 authors at 6 institutions in 1 country.

Wei-Hung Chen *Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.ORCID 0000-0003-3724-9195
Agnes Hajduczki *Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Elizabeth J MartinezEmerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.ORCID 0000-0002-7755-7056
Hongjun BaiEmerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.ORCID 0000-0002-3501-3974
Hanover MatzDepartment of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD, USA.
Thomas M HillDepartment of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD, USA.
Eric LewitusEmerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
William C ChangEmerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.ORCID 0000-0003-4967-6426
Layla DawitEmerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Caroline E PetersonEmerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Phyllis A ReesEmerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Adelola B AjayiEmerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Emily S GolubEmerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Isabella SwaffordEmerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.ORCID 0000-0001-8638-2772
Vincent DussuptEmerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.ORCID 0000-0002-8579-6356
Sapna DavidEmerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Sandra V MayerHenry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.
Sandrine SomanViral Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Caitlin KuklisViral Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.ORCID 0000-0002-9974-6640
Courtney CorbittEmerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Jocelyn KingEmerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.ORCID 0000-0003-3348-1632
Misook ChoeEmerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Rajeshwer S SankhalaEmerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Paul V ThomasEmerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Michelle ZemilU.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA.ORCID 0000-0002-1700-6658
Lindsay WieczorekHenry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.
Tricia HartTrudeau Institute, Saranac Lake, NY, USA.
Debora DusoTrudeau Institute, Saranac Lake, NY, USA.
Larry KummerTrudeau Institute, Saranac Lake, NY, USA.
Lianying YanDepartment of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA.
Spencer L SterlingDepartment of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA.
Eric D LaingDepartment of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA.
Christopher C BroderDepartment of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA.ORCID 0000-0001-5382-0510
Jazmean K WilliamsIntegral Molecular, Philadelphia, PA, USA.
Edgar DavidsonIntegral Molecular, Philadelphia, PA, USA.
Benjamin J DoranzIntegral Molecular, Philadelphia, PA, USA.ORCID 0000-0002-8245-3083
Shelly J KrebsEmerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.ORCID 0000-0003-1136-1760
Victoria R PolonisU.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Dominic Paquin-ProulxEmerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.ORCID 0000-0003-1407-3414
Morgane RollandEmerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.ORCID 0000-0003-3650-8490
William W ReileyTrudeau Institute, Saranac Lake, NY, USA.
Gregory D GromowskiViral Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.ORCID 0000-0003-4576-4277
Kayvon ModjarradEmerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Helen DooleyDepartment of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD, USA. hdooley@som.umaryland.edu.ORCID 0000-0002-2570-574X
M Gordon JoyceEmerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA. gjoyce@eidresearch.org.ORCID 0000-0002-6808-7232
Henry M. Jackson Foundation · USWalter Reed Army Institute of Research · USTrudeau Institute · USUniformed Services University of the Health Sciences · USIntegral Molecular (United States) · USUniversity of Maryland, Baltimore · US

Funding

User Training and OutreachP30GM124165 · NIGMS · CORNELL UNIVERSITY · PI STEVEN E EALICK · 2018 to 2026
$34.2M
Pixel Array Detector for Macromolecular CrystallographyS10OD021527 · OD · CORNELL UNIVERSITY · PI EALICK, STEVEN E · 2016 to 2016
$2.0M
NIGMS NIH HHS P30 GM124165NIH HHS S10 OD021527
6 · The paper itself

Abstract

Despite rapid and ongoing vaccine and therapeutic development, SARS-CoV-2 continues to evolve and evade, presenting a need for next-generation diverse therapeutic modalities. Here we show that nurse sharks immunized with SARS-CoV-2 recombinant receptor binding domain (RBD), RBD-ferritin (RFN), or spike protein ferritin nanoparticle (SpFN) immunogens elicit a set of new antigen receptor antibody (IgNAR) molecules that target two non-overlapping conserved epitopes on the spike RBD. Representative shark antibody variable NAR-Fc chimeras (ShAbs) targeting either of the two epitopes mediate cell-effector functions, with high affinity to all SARS-CoV-2 viral variants of concern, including the divergent Omicron strains. The ShAbs potently cross-neutralize SARS-CoV-2 WA-1, Alpha, Beta, Delta, Omicron BA.1 and BA.5, and SARS-CoV-1 pseudoviruses, and confer protection against SARS-CoV-2 challenge in the K18-hACE2 transgenic mouse model. Structural definition of the RBD-ShAb01-ShAb02 complex enabled design and production of multi-specific nanobodies with enhanced neutralization capacity, and picomolar affinity to divergent sarbecovirus clade 1a, 1b and 2 RBD molecules. These shark nanobodies represent potent immunotherapeutics both for current use, and future sarbecovirus pandemic preparation.

Indexed as

COVID-19Severe acute respiratory syndrome-related coronavirusSingle-Domain AntibodiesAnimalsAntibodies, NeutralizingAntibodies, ViralEpitopesFerritinsImmunoglobulin Fc FragmentsMiceMice, TransgenicSARS-CoV-2SharksSpike Glycoprotein, CoronavirusAntibodies, NeutralizingAntibodies, ViralEpitopesFerritinsImmunoglobulin Fc FragmentsSingle-Domain AntibodiesSpike Glycoprotein, Coronavirusspike protein, SARS-CoV-2

Identifiers

PMID36737435
PMCPMC9896449
OpenAlexW4319159419

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.