Evidence map›Paper›PMID 36736323›Full record

ArticleCurrent biology : CB2023

Multi-site phosphorylation of yeast Mif2/CENP-C promotes inner kinetochore assembly.

Stephen M Hinshaw, Yun Quan, Jiaxi Cai, Ann L Zhou, Huilin Zhou

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Current biology : CB, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
2.1field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 10 citations in OpenAlex.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Stephen M HinshawStanford Cancer Institute, Stanford School of Medicine, 1291 Welch Road, Stanford, CA 94305, USA. Electronic address: hinshaw@stanford.edu.
Yun QuanDepartment of Cellular and Molecular Medicine, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92039, USA.
Jiaxi CaiDepartment of Cellular and Molecular Medicine, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92039, USA.
Ann L ZhouDepartment of Cellular and Molecular Medicine, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92039, USA.
Huilin ZhouDepartment of Cellular and Molecular Medicine, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92039, USA. Electronic address: huzhou@health.ucsd.edu.
University of California San Diego · USStanford Medicine · US

Funding

Suppression of duplication-mediated genome rearrangements by protein sumoylationR01GM116897 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ZHOU, HUILIN · 2015 to 2023
$2.6M
Orbitrap Fusion Mass SpectrometerS10OD023498 · OD · LUDWIG INSTITUTE FOR CANCER RES LTD · PI ZHOU, HUILIN · 2017 to 2017
$600k
NIGMS NIH HHS R01 GM116897NIH HHS S10 OD023498
6 · The paper itself

Abstract

Kinetochores control eukaryotic chromosome segregation by connecting chromosomal centromeres to spindle microtubules. Duplication of centromeric DNA necessitates kinetochore disassembly and subsequent reassembly on nascent sisters. To search for a regulatory mechanism that controls the earliest steps of this process, we studied Mif2/CENP-C, an essential basal component of the kinetochore. We found that phosphorylation of a central region of Mif2 (Mif2-PEST) enhances inner kinetochore assembly. Eliminating Mif2-PEST phosphorylation sites progressively impairs cellular fitness. The most severe Mif2-PEST mutations are lethal in cells lacking otherwise non-essential inner kinetochore factors. These data show that multi-site phosphorylation of Mif2/CENP-C controls inner kinetochore assembly.

Indexed as

KinetochoresSaccharomyces cerevisiae ProteinsCentromereCentromere Protein AChromosomal Proteins, Non-HistoneDNA-Binding ProteinsMitosisPhosphorylationSaccharomyces cerevisiaeCentromere Protein Acentromere protein CChromosomal Proteins, Non-HistoneDNA-Binding ProteinsMIF2 protein, S cerevisiaeSaccharomyces cerevisiae Proteinscell cyclecentromerechromosome segregationkinasekinetochoremitosisphosphorylation

Identifiers

PMID36736323
PMCPMC9992315
OpenAlexW4318988369

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.