Evidence map›Paper›PMID 36734141›Full record

ArticleHistology and histopathology2023

Correlation of 3'-phosphoadenosine-5'-phosphosulfate synthase 1 (PAPSS1) expression with clinical parameters and prognosis in esophageal squamous cell carcinoma.

Xuyou Zhu, Peipei Zhu, Xue Chen, Long Zhang, Caixia Wu, Haoyang Zhang, Xiaoying Shen, Yao Qi, Mingmin Chen, Shunli Wang and 1 more

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Article in Histology and histopathology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

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4 · The record

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5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Xuyou Zhu *Department of Pathology, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China.
Peipei Zhu *Department of Pathology, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China.
Xue ChenDepartment of Pathology, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China.
Long ZhangDepartment of Pathology, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China.
Caixia WuDepartment of Pathology, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China.
Haoyang ZhangDepartment of Pathology, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China.
Xiaoying ShenShanghai OUTDO Biotech Co. Ltd. Shanghai, China.
Yao QiShanghai OUTDO Biotech Co. Ltd. Shanghai, China.
Mingmin ChenShanghai OUTDO Biotech Co. Ltd. Shanghai, China.
Shunli WangDepartment of Pathology, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China. shunli_wang@sina.com.
Xianghua YiDepartment of Pathology, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China. yixhxf@163.com.
Tongji University · CNViva Biotech (China) · CN

Funding

Fundamental Research Funds for the Central Universities 22120220609Natural Science Foundation of Shanghai 16ZR1432100Natural Science Foundation of Shanghai 19ZR1448500Shanghai Health Bureau Key Special Fund for Medicine 20134034Shanghai Science and Technology Commission Medical Guidance Project Fund 19411964700Trust of the National Natural Science Foundation of China 81802769Trust of the National Natural Science Foundation of China 82170082
6 · The paper itself

Abstract

backgroundIn recent years, 3'-phosphoadenosine-5'-phosphosulfate synthase 1 (PAPSS1) has been found to be highly expressed in some cancers and significantly associated with prognosis. Nevertheless, the role of PAPSS1 in esophageal squamous cell carcinoma (ESCC) is poorly understood.

methodsIn this study, PAPSS1 expression in ESCC samples was researched through real-time quantitative polymerase chain reaction (qPCR), immunohistochemistry (IHC), and western blot (WB) techniques. siRNA technology was then used to inhibit PAPSS1 expression in ESCC cells, and cytologic tests were conducted to research gene affection on cell apoptosis, proliferation, and migration. Then, the expression of Bcl2, Ki67, and Snail was detected using qPCR and WB tests. These experimental data were analyzed by GraphPad software, where the P-value<0.05 was statistically significant.

resultsThe results showed that PAPSS1 expression level in ESCC tissues was higher than in the adjacent tissues. The data also showed that PAPSS1 was significantly correlated with N stage, and that the patients with high expressions had longer survival time. After transfection for 48 hours, the cell apoptosis rate of siRNA-PAPSS1 transfected groups decreased significantly, whereas the cell proliferation rate and migration ability increased relative to the control. At the same time, the expression levels of Bcl2, Ki67 and Snail were all upregulated by siRNA-PAPSS1. PAPSS1, however, was suppressed.

conclusionsPAPSS1 may be an ESCC suppressor gene, and its specific molecular mechanism in ESCC needs to be further studied.

Indexed as

Esophageal NeoplasmsEsophageal Squamous Cell CarcinomaCell Line, TumorCell MovementCell ProliferationGene Expression Regulation, NeoplasticHumansKi-67 AntigenMultienzyme ComplexesPrognosisProto-Oncogene Proteins c-bcl-2RNA, Small InterferingSulfate AdenylyltransferaseKi-67 AntigenMultienzyme ComplexesPAPS synthetaseProto-Oncogene Proteins c-bcl-2RNA, Small InterferingSulfate Adenylyltransferase

Identifiers

PMID36734141
OpenAlexW4319063560

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.