Evidence map›Paper›PMID 36733723›Full record

ArticleBiomedical optics express2022

Increasing the field-of-view in oblique plane microscopy via optical tiling.

Bingying Chen, Bo-Jui Chang, Felix Y Zhou, Stephan Daetwyler, Etai Sapoznik, Benjamin A Nanes, Isabella Terrazas, Gabriel M Gihana, Lizbeth Perez Castro, Isaac S Chan and 5 more

Open access · goldAbstract read
In one paragraph

Article in Biomedical optics express, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
5.5field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 24 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Active Remote Focus Stabilization in Oblique Plane Microscopy.bioRxiv : the preprint server for biology · 2024
    Article
  13. Article
  14. Article
  15. Adaptive optics in an oblique plane microscope.Biomedical optics express · 2024
    Article
  16. Article
  17. Article
  18. Adaptive Optics in an Oblique Plane Microscope.bioRxiv : the preprint server for biology · 2024
    Article
  19. Review
  20. Mesoscopic Oblique Plane Microscopy via Light-sheet Mirroring.bioRxiv : the preprint server for biology · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 2 institutions in 1 country.

Bingying ChenLyda Hill Department of Bioinformatics, UT Southwestern Medical Center, Dallas, Texas, USA.
Bo-Jui ChangLyda Hill Department of Bioinformatics, UT Southwestern Medical Center, Dallas, Texas, USA.ORCID https://orcid.org/0000-0002-5513-7106
Felix Y ZhouLyda Hill Department of Bioinformatics, UT Southwestern Medical Center, Dallas, Texas, USA.
Stephan DaetwylerLyda Hill Department of Bioinformatics, UT Southwestern Medical Center, Dallas, Texas, USA.
Etai SapoznikLyda Hill Department of Bioinformatics, UT Southwestern Medical Center, Dallas, Texas, USA.
Benjamin A NanesLyda Hill Department of Bioinformatics, UT Southwestern Medical Center, Dallas, Texas, USA.
Isabella TerrazasDepartment of Internal Medicine, Division of Hematology and Oncology, UT Southwestern Medical Center, Dallas, Texas, USA.
Gabriel M GihanaLyda Hill Department of Bioinformatics, UT Southwestern Medical Center, Dallas, Texas, USA.
Lizbeth Perez CastroDepartment of Cell Biology, UT Southwestern Medical Center, Dallas, Texas, USA.
Isaac S ChanDepartment of Internal Medicine, Division of Hematology and Oncology, UT Southwestern Medical Center, Dallas, Texas, USA.
Maralice Conacci-SorrellDepartment of Cell Biology, UT Southwestern Medical Center, Dallas, Texas, USA.
Kevin M DeanLyda Hill Department of Bioinformatics, UT Southwestern Medical Center, Dallas, Texas, USA.ORCID https://orcid.org/0000-0003-0839-2320
Alfred Millett-SikkingCalico Life Sciences LLC, South San Francisco, CA, USA.
Andrew G YorkCalico Life Sciences LLC, South San Francisco, CA, USA.
Reto FiolkaLyda Hill Department of Bioinformatics, UT Southwestern Medical Center, Dallas, Texas, USA.
Southwestern Medical Center · USEnzo Life Sciences (United States) · US

Funding

Technical Development Unit 2: Intelligent Hyperspectral Imaging of Subcellular Molecular States at the Whole Organ LevelU54CA268072 · NCI · UT SOUTHWESTERN MEDICAL CENTER · PI Reto Paul Fiolka · 2021 to 2026
$9.1M
Transformative microscopes to image across spatiotemporal scalesR35GM133522 · NIGMS · UT SOUTHWESTERN MEDICAL CENTER · PI Reto Paul Fiolka · 2019 to 2026
$3.1M
Dermatology Research Training ProgramT32AR065969 · NIAMS · UT SOUTHWESTERN MEDICAL CENTER · PI PONCIANO D CRUZ, Tamia Alisha Harris-Tryon · 2014 to 2026
$1.6M
NCI NIH HHS U54 CA268072NIAMS NIH HHS T32 AR065969NIGMS NIH HHS R35 GM133522
6 · The paper itself

Abstract

Fast volumetric imaging of large fluorescent samples with high-resolution is required for many biological applications. Oblique plane microscopy (OPM) provides high spatiotemporal resolution, but the field of view is typically limited by its optical train and the pixel number of the camera. Mechanically scanning the sample or decreasing the overall magnification of the imaging system can partially address this challenge, albeit by reducing the volumetric imaging speed or spatial resolution, respectively. Here, we introduce a novel dual-axis scan unit for OPM that facilitates rapid and high-resolution volumetric imaging throughout a volume of 800 × 500 × 200 microns. This enables us to perform volumetric imaging of cell monolayers, spheroids and zebrafish embryos with subcellular resolution. Furthermore, we combined this microscope with a multi-perspective projection imaging technique that increases the volumetric interrogation rate to more than 10 Hz. This allows us to rapidly probe a large field of view in a dimensionality reduced format, identify features of interest, and volumetrically image these regions with high spatiotemporal resolution.

Identifiers

PMID36733723
PMCPMC9872888
OpenAlexW4295219632

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.