ArticlePlant physiology2023
E3 ligase BRG3 persulfidation delays tomato ripening by reducing ubiquitination of the repressor WRKY71.
Article in Plant physiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 45 citations in OpenAlex.
- WRKY transcription factors in horticultural crops: regulating quality traits and fruit stress responses.Planta · 2026Review
- Hydrogen sulfide-mediated persulfidation of ERF106 controls pear fruit aroma biosynthesis via disrupting the ERF106-MYB21-FAD8 complex.The Plant journal : for cell and molecular biology · 2026Article
- Protein persulfidation in plants: a central regulator of multiple signaling pathways.Plant cell reports · 2026Review
- HPlant, cell & environment · 2026Article
- Protein persulfidation: a ubiquitous modification regulating a broad spectrum of biological processes.Journal of experimental botany · 2026Review
- Positive regulatory role of EuWRKY1 in the biosynthesis of Eucommia ulmoides rubber.Functional & integrative genomics · 2026Article
- 'Candidatus Liberibacter Asiaticus' Effector SECP8 Subverts Salicylic Acid-Mediated Citrus Immunity via a Two-Pronged Impairment of CsTCP15 Dimerization.Plant biotechnology journal · 2026Article
- The carotenoids metabolism during tomato fruit ripening: insights into multi-level regulatory network.BMC plant biology · 2026Review
- Protein post-translational modifications: key switches coordinating fruit ripening regulatory networks.Horticulture research · 2026Article
- Salicylic acid reduces MdPUB24-mediated ubiquitination of MdWRKY40 to suppress ethylene biosynthesis in apple fruit.Horticulture research · 2026Article
- From odor to order: unveiling the crucial role of hydrogen sulfide in plant life.Horticulture research · 2026Article
- Alfalfa Transcription Factor MsNAC2a Orchestrates the Homeostasis of Salt Stress Responses via Reactive Oxygen Species Accumulation and Hydrogen Sulphide Depletion.Plant biotechnology journal · 2025Article
- WRKYs as regulatory hubs of secondary metabolic networks: Diverse inducers and distinct responses.Plant communications · 2025Review
- Functional interactions among H2O2, NO, H2S, and melatonin in the physiology, metabolism, and quality of horticultural Solanaceae.Journal of experimental botany · 2025Review
- VaMIEL1-mediated ubiquitination of VaMYB4a orchestrates cold tolerance through integrated transcriptional and oxidative stress pathways in grapevine.Horticulture research · 2025Article
- Decoding Plant-Pathogen Interactions: A Comprehensive Exploration of Effector-Plant Transcription Factor Dynamics.Molecular plant pathology · 2025Review
- Post-Translational Modification of WRKY Transcription Factors.Plants (Basel, Switzerland) · 2024Review
- Article
- Dynamic interplay of reactive oxygen and nitrogen species (ROS and RNS) in plant resilience: unveiling the signaling pathways and metabolic responses to biotic and abiotic stresses.Plant cell reports · 2024Review
- Persulfidome of Sweet Pepper Fruits during Ripening: The Case Study of Leucine Aminopeptidase That Is Positively Modulated by HAntioxidants (Basel, Switzerland) · 2024Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hydrogen sulfide (H2S) is a gaseous signaling molecule reported to play multiple roles in fruit ripening. However, the molecular mechanisms underlying H2S-mediated delay in fruit ripening remain to be established. Here, the gene encoding a WRKY transcription factor, WRKY71, was identified as substantially upregulated in H2S-treated tomato (Solanum lycopersicum) via transcriptome profiling. The expression of WRKY71 was negatively associated with that of CYANOALANINE SYNTHASE1 (CAS1). Transient and stable genetic modification experiments disclosed that WRKY71 acts as a repressor of the tomato ripening process. CAS1 appears to play an opposite role, based on the finding that the ripening process was delayed in the cas1 mutant and accelerated in CAS1-OE tomatoes. Dual-luciferase reporter assay, yeast one-hybrid, electrophoretic mobility shift assay, and transient transformation experiments showed that WRKY71 bound to the CAS1 promoter and suppressed its activation. Moreover, the persulfidation of WRKY71 enhanced its binding ability to the CAS1 promoter. Data from luciferase complementation and Y2H assays confirmed that WRKY71 interacts with a BOI-related E3 ubiquitin-protein ligase 3 (BRG3) and is ubiquitinated in vitro. Further experiments showed that modification of BRG3 via persulfidation at Cys206 and Cys212 led to reduced ubiquitination activity. Our findings support a model whereby BRG3 undergoes persulfidation at Cys206 and Cys212, leading to reduced ubiquitination activity and decreased interactions with the WRKY71 transcript, with a subsequent increase in binding activity of the persulfidated WRKY71 to the CAS1 promoter, resulting in its transcriptional inhibition and thereby delayed ripening of tomatoes. Our collective findings provide insights into a mechanism of H2S-mediated regulation of tomato fruit ripening.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.