ArticlePlant physiology2023
ETHYLENE RESPONSE FACTORS 4.1/4.2 with an EAR motif repress anthocyanin biosynthesis in red-skinned pears.
Article in Plant physiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 48 citations in OpenAlex.
- Multifaceted mechanisms underlying species- and cultivar-associated differential anthocyanin accumulation in fruits.Horticulture research · 2026Article
- Ethylene-responsive CmERF3-CmMYB4 cascade suppresses anthocyanin biosynthesis inHorticulture research · 2026Article
- Transcriptional Regulation of the Novel Theacrine Synthase Gene CsTcS2 by the CsTINY-CsWRKY33 Module Underpins Theacrine Biosynthesis in Camellia sinensis.Plant biotechnology journal · 2026Article
- Identification and analysis of the AP2/ERF gene family inFrontiers in plant science · 2026Article
- The Mechanism of TT2-TypePlants (Basel, Switzerland) · 2025Article
- Identification of regulatory genes involved in anthocyanin accumulation in radish leaves using bulk segregant analysis.Scientific reports · 2025Article
- A novel function for the transcription factor sensitive to proton rhizotoxicity1 in promoting anthocyanin accumulation in strawberry.Plant biotechnology journal · 2025Article
- Study on the dynamics of fruits color related genes and metabolites during the fruit development of Zanthoxylum bungeanum 'Hanyuan'.BMC plant biology · 2025Article
- Improving the Surface Color and Delaying Softening of Peach by Minimizing the Harmful Effects of Ethylene in the Package.Foods (Basel, Switzerland) · 2025Article
- Effects of different hormones on the color of tree peony leaves.BMC plant biology · 2025Article
- Combined Multi-Omics and Co-Expression Network Analyses Uncover the Pigment Accumulation Mechanism of Orange-Red Petals inBiology · 2025Article
- Phosphorylation of the strawberry MADS-box CMB1 regulates ripening via the catabolism of abscisic acid.The New phytologist · 2025Article
- Integrated metabolomic and transcriptomic analysis of anthocyanin metabolism in wheat pericarp.BMC genomic data · 2025Article
- Genome-wide identification and analysis of ERF transcription factors related to abiotic stress responses in Nelumbo nucifera.BMC plant biology · 2024Article
- Phylogenomic Analysis and Functional Characterization of the APETALA2/Ethylene-Responsive Factor Transcription Factor Across Solanaceae.International journal of molecular sciences · 2024Article
- The R2R3 MYB Ruby1 is activated by two cold responsive ethylene response factors, via the retrotransposon in its promoter, to positively regulate anthocyanin biosynthesis in citrus.The Plant journal : for cell and molecular biology · 2024Article
- An 'activator-repressor' loop controls the anthocyanin biosynthesis in red-skinned pear.Molecular horticulture · 2024Article
- Allelic variation of BBX24 is a dominant determinant controlling red coloration and dwarfism in pear.Plant biotechnology journal · 2024Article
- Transcriptomic study of the role of MeFtsZ2-1 in pigment accumulation in cassava leaves.BMC genomics · 2024Article
- The miR156/SPL12 module orchestrates fruit colour change through directly regulating ethylene production pathway in blueberry.Plant biotechnology journal · 2024Article
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5 authors at 2 institutions in 1 country.
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Abstract
Red-skinned pears (Pyrus L.) are preferred to consumers for their attractive color and abundant anthocyanins. Pyrus ETHYLENE RESPONSE FACTOR 3 (PyERF3) positively regulates anthocyanin biosynthesis through interacting with Pyrus myeloblastosis family 114 (PyMYB114) and Pyrus basic helix-loop-helix 3 (PybHLH3) in red-skinned pears. However, the role of APETALA2/ethylene response factors (AP2/ERFs), which negatively regulate anthocyanin biosynthesis, remains unclear in red-skinned pears. Here, we validated that 2 AP2/ERFs, PyERF4.1 and PyERF4.2, screened from the transcriptome data of 'Starkrimson' pear (Pyrus communis L.) and its green mutant, inhibit anthocyanin biosynthesis in transgenic pear calli, as well as in overexpression and gene-edited tomato (Solanum lycopersicum) fruits. Meanwhile, the co-transformation of PyERF4.1/PyERF4.2 with PyERF3-PyMYB114-PybHLH3 inhibited anthocyanin biosynthesis in pear fruits and strawberry (Fragaria vesca) receptacles. Further assays showed that PyMYB114 activated the transcription of PyERF4.1/PyERF4.2; PyERF4.1/PyERF4.2 then interacted with PyERF3 to affect the stability of the PyERF3-PyMYB114-PybHLH3 complex, thereby inhibiting the transcription of the anthocyanin biosynthesis gene Pyrus anthocyanidin synthase (PyANS). Furthermore, deletion of the ERF-associated-amphiphilic repression (EAR) motif eliminated the inhibitory effect of PyERF4.1/PyERF4.2 on anthocyanin biosynthesis, and a mutation of the PyERF4.2-EAR motif (LxLxM to LxLxL) strengthened the inhibitory effect, demonstrating that the EAR motif is indispensable for the inhibitory effect of PyERF4.1/PyERF4.2 on anthocyanin biosynthesis in pears. Our study has shed light on a feedback regulatory loop mechanism that balances the excessive accumulation of anthocyanins in red-skinned pears, providing insights into the regulatory mechanism of anthocyanin biosynthesis and the regulatory network of coloration in red-skinned pears.
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