Evidence map›Paper›PMID 36727532›Full record

ArticleJournal of cell science2023

Fast4DReg - fast registration of 4D microscopy datasets.

Joanna W Pylvänäinen, Romain F Laine, Bruno M S Saraiva, Sujan Ghimire, Gautier Follain, Ricardo Henriques, Guillaume Jacquemet

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Article in Journal of cell science, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

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  16. Live-cell imaging in the deep learning era.Current opinion in cell biology · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Joanna W PylvänäinenÅbo Akademi University, Faculty of Science and Engineering, Biosciences, Turku 20520, Finland.
Romain F LaineMRC Laboratory for Molecular Cell Biology, University College London, London WC1E 6BT, UK.
Bruno M S SaraivaInstituto Gulbenkian de Ciência, Oeiras 2780-156, Portugal.
Sujan GhimireÅbo Akademi University, Faculty of Science and Engineering, Biosciences, Turku 20520, Finland.
Gautier FollainÅbo Akademi University, Faculty of Science and Engineering, Biosciences, Turku 20520, Finland.
Ricardo HenriquesInstituto Gulbenkian de Ciência, Oeiras 2780-156, Portugal.
Guillaume JacquemetÅbo Akademi University, Faculty of Science and Engineering, Biosciences, Turku 20520, Finland.ORCID 0000-0002-9286-920X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Unwanted sample drift is a common issue that plagues microscopy experiments, preventing accurate temporal visualization and quantification of biological processes. Although multiple methods and tools exist to correct images post acquisition, performing drift correction of three-dimensional (3D) videos using open-source solutions remains challenging and time consuming. Here, we present a new tool developed for ImageJ or Fiji called Fast4DReg that can quickly correct axial and lateral drift in 3D video-microscopy datasets. Fast4DReg works by creating intensity projections along multiple axes and estimating the drift between frames using two-dimensional cross-correlations. Using synthetic and acquired datasets, we demonstrate that Fast4DReg can perform better than other state-of-the-art open-source drift-correction tools and significantly outperforms them in speed. We also demonstrate that Fast4DReg can be used to register misaligned channels in 3D using either calibration slides or misaligned images directly. Altogether, Fast4DReg provides a quick and easy-to-use method to correct 3D imaging data before further visualization and analysis.

Indexed as

Imaging, Three-DimensionalMicroscopyMicroscopy, Video3D drift correctionFijiImage analysisImageJLive imaging

Identifiers

PMID36727532
PMCPMC10022679

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.