ArticleEnvironmental epigenetics2023
Effects of paternal methionine supplementation on sperm DNA methylation and embryo transcriptome in sheep.
Article in Environmental epigenetics, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 20 citations in OpenAlex.
- Across species identification of genes bridging neurological and reproductive functions in mammals.iScience · 2026Article
- Paternal Methionine Supplementation Alters DNA Methylation Patterns in Preimplantation Sheep Embryos.Epigenomes · 2026Article
- Germline and Embryonic Mechanisms in the Epigenetic Inheritance of Neurodevelopmental and Cognitive Traits in Mammals.Biomolecules · 2026Review
- Sex-specific multigenerational epigenetic responses to real-world chemical mixture exposure in an outbred sheep model.bioRxiv : the preprint server for biology · 2026Article
- Tributyltin chloride alters the structural, genomic, and epigenomic integrity of postejaculatory mammalian sperm.Epigenetics · 2025Article
- Epigenetic remodeling of sheep oocytes and embryos induced by maternal methionine supplementation.Epigenetics · 2025Article
- Transgenerational skin adaptations to late-gestation heat stress in great-granddaughters.JDS communications · 2025Article
- Molecular Genetic Basis of Reproductive Fitness in Tibetan Sheep on the Qinghai-Tibet Plateau.Genes · 2025Article
- Transgenerational Epigenetic and Phenotypic Inheritance Across Five Generations in Sheep.International journal of molecular sciences · 2025Article
- Late Gestation Maternal Nutrition Has a Stronger Impact on Offspring Liver Transcriptome than Full-Gestation Supplementation in Beef Cattle.Veterinary sciences · 2025Article
- Comparative transcriptome analysis of bull X- and Y-spermatozoa.Scientific reports · 2025Article
- Methionine supplementation-induced alteration of sheep seminal plasma miRNAs and proteome.Journal of animal science · 2025Article
- Characterization of genomic regions escaping epigenetic reprogramming in sheep.Environmental epigenetics · 2024Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Environmental effects on gene expression and offspring development can be mediated by epigenetic modifications. It is well established that maternal diet influences DNA methylation patterns and phenotypes in the offspring; however, the epigenetic effects of paternal diet on developing offspring warrants further investigation. Here, we examined how a prepubertal methionine-enriched paternal diet affected sperm DNA methylation and its subsequent effects on embryo gene expression. Three treatment and three control rams were bred to seven ewes, and blastocysts were flushed for RNA extraction. Semen was collected from all rams and submitted for reduced representation bisulfite sequencing analysis. In total, 166 differentially methylated cytosines were identified in the sperm from treatment versus control rams. Nine genes were found to be differentially expressed in embryos produced from treatment versus control rams, and seven differentially methylated cytosines in the sperm were found to be highly correlated with gene expression in the embryos. Our results demonstrate that sperm methylation differences induced by diet may influence fetal programming.
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