Evidence map›Paper›PMID 36726680›Full record

ArticleFrontiers in plant science2022

Downregulation of barley ferulate 5-hydroxylase dramatically alters straw lignin structure without impact on mechanical properties.

Reza Shafiei, Matthew Hooper, Christopher McClellan, Helena Oakey, Jennifer Stephens, Catherine Lapierre, Yukiko Tsuji, Geert Goeminne, Ruben Vanholme, Wout Boerjan and 2 more

Abstract read
In one paragraph

Article in Frontiers in plant science, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Review
  5. GM crops & food · 2024
    Article
  6. Article
  7. Review
  8. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Reza ShafieiDivision of Plant Sciences, School of Life Sciences, University of Dundee at the James Hutton Institute, Dundee, United Kingdom.
Matthew HooperDivision of Plant Sciences, School of Life Sciences, University of Dundee at the James Hutton Institute, Dundee, United Kingdom.
Christopher McClellanDivision of Plant Sciences, School of Life Sciences, University of Dundee at the James Hutton Institute, Dundee, United Kingdom.
Helena OakeyDivision of Plant Sciences, School of Life Sciences, University of Dundee at the James Hutton Institute, Dundee, United Kingdom.
Jennifer StephensCell And Molecular Sciences, James Hutton Institute, Dundee, United Kingdom.
Catherine LapierreINRAE AgroParisTech, Université Paris Saclay, IJPB, Versailles, France.
Yukiko TsujiDepartment of Biochemistry, University of Wisconsin-Madison, Madison, WI, United States.
Geert GoeminneVIB-UGent, Metabolomics Core, Ghent, Belgium.
Ruben VanholmeDepartment of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium.
Wout BoerjanDepartment of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium.
John RalphDepartment of Energy's Great Lakes Bioenergy Research Center, The Wisconsin Energy Institute, University of Wisconsin-Madison, Madison, WI, United States.
Claire HalpinDivision of Plant Sciences, School of Life Sciences, University of Dundee at the James Hutton Institute, Dundee, United Kingdom.

Funding

Biotechnology and Biological Sciences Research Council BB/G016232/1
6 · The paper itself

Abstract

Barley is a major cereal crop for temperate climates, and a diploid genetic model for polyploid wheat. Cereal straw biomass is an attractive source of feedstock for green technologies but lignin, a key determinant of feedstock recalcitrance, complicates bio-conversion processes. However, manipulating lignin content to improve the conversion process could negatively affect agronomic traits. An alternative approach is to manipulate lignin composition which influences the physical and chemical properties of straw. This study validates the function of a barley ferulate 5-hydroxylase gene and demonstrates that its downregulation using the RNA-interference approach substantially impacts lignin composition. We identified five barley genes having putative ferulate 5-hydroxylase activity. Downregulation of

Indexed as

barleybioeconomybiosynthetic pathwayferulate 5-hydroxylaseligninRNAiS/Gstraw

Identifiers

PMID36726680
PMCPMC9886061

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.