ArticlePlant methods2023
An optimized pipeline for live imaging whole Arabidopsis leaves at cellular resolution.
Article in Plant methods, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Growth-rate coordination across the width of a leaf preserves its flatness.PLoS biology · 2026Article
- Growth history leaves a geometric trace in puzzle cells.EMBO reports · 2026Article
- Technological advances in visualizing and rewiring microtubules during plant development.Journal of experimental botany · 2025Review
- A space for time. Exploring temporal regulation of plant development across spatial scales.The Plant journal : for cell and molecular biology · 2025Review
- A Protocol for Live Imaging of Arabidopsis Gynoecium Development Using Confocal Microscopy.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Modulation of cell differentiation and growth underlies the shift from bud protection to light capture in cauline leaves.Plant physiology · 2024Article
- An optimized live imaging and growth analysis approach for Arabidopsis Sepals.bioRxiv : the preprint server for biology · 2024Article
- The Arabidopsis leaf quantitative atlas: a cellular and subcellular mapping through unified data integration.Quantitative plant biology · 2024Article
- An optimized live imaging and multiple cell layer growth analysis approach using Arabidopsis sepals.Frontiers in plant science · 2024Article
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Authors and funding
2 authors.
Funding
Abstract
backgroundLive imaging is the gold standard for determining how cells give rise to organs. However, tracking many cells across whole organs over large developmental time windows is extremely challenging. In this work, we provide a comparably simple method for confocal live imaging entire Arabidopsis thaliana first leaves across early development. Our imaging method works for both wild-type leaves and the complex curved leaves of the jaw-1D mutant.
resultsWe find that dissecting the cotyledons, affixing a coverslip above the samples and mounting samples with perfluorodecalin yields optimal imaging series for robust cellular and organ level analysis. We provide details of our complementary image processing steps in MorphoGraphX software for segmenting, tracking lineages, and measuring a suite of cellular properties. We also provide MorphoGraphX image processing scripts we developed to automate analysis of segmented images and data presentation.
conclusionsOur imaging techniques and processing steps combine into a robust imaging pipeline. With this pipeline we are able to examine important nuances in the cellular growth and differentiation of jaw-D versus WT leaves that have not been demonstrated before. Our pipeline is approachable and easy to use for leaf development live imaging.
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