ArticleScience China. Life sciences2023
DEtail-seq is an ultra-efficient and convenient method for meiotic DNA break profiling in multiple organisms.
Article in Science China. Life sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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8 citing papers in PubMed, 5 citations in OpenAlex.
- Mapping Meiotic Recombination DNA Double-Strand Breaks (DSBs) Hotspots -Methodological Advances and Challenges.Advanced genetics (Hoboken, N.J.) · 2026Article
- Landscape of plastid DNA breaks in Arabidopsis during development and environmental stimulus.The Plant cell · 2026Article
- Decoding DNA metabolism and its clinical relevance through the lens of high-throughput sequencing assays.Medical review (2021) · 2026Review
- Multifactorial and multiplexed epigenomic profiling of five DNA modifications in plants with mDIP-seq.Science China. Life sciences · 2026Article
- PEN1 catalyses RNA primer removal during plastid DNA replication in maize.Nature plants · 2025Article
- METTL16 is Required for Meiotic Sex Chromosome Inactivation and DSB Formation and Recombination during Male Meiosis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- siqRNA-seq is a spike-in-independent technique for quantitative mapping of mRNA landscape.BMC genomics · 2024Article
- Primase promotes the competition between transcription and replication on the same template strand resulting in DNA damage.Nature communications · 2024Article
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Authors and funding
9 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Programmed DNA double-strand break (DSB) formation is a crucial step in meiotic recombination, yet techniques for high-efficiency and precise mapping of the 3' ends of DSBs are still in their infancy. Here, we report a novel technique, named DNA End tailing and sequencing (DEtail-seq), which can directly and ultra-efficiently characterize the 3' ends of meiotic DSBs with near single-nucleotide resolution in a variety of species, including yeast, mouse, and human. We find that the 3' ends of meiotic DSBs are stable without significant resection in budding yeast. Meiotic DSBs are strongly enriched in de novo H3K4me3 peaks in the mouse genome at leptotene stage. We also profile meiotic DSBs in human and find DSB hotspots are enriched near the common fragile sites during human meiosis, especially at CCCTC-binding factor (CTCF)-associated enhancers. Therefore, DEtail-seq provides a powerful method to detect DSB ends in various species, and our results provide new insights into the distribution and regulation of meiotic DSB hotspots.
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