ArticleApplied biochemistry and biotechnology2023
Efficient Expression of Candida antarctica Lipase B in Pichia pastoris and Its Application in Biodiesel Production.
Article in Applied biochemistry and biotechnology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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6 citing papers in PubMed, 16 citations in OpenAlex.
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- Biodiesel Production from Soybean Oil Using a Free-Enzyme and Whole-Cell Dual Lipase System as a Biocatalyst.Applied biochemistry and biotechnology · 2025Article
- Effect of Fold-Promoting Mutation and Signal Peptide Screening on Recombinant Glucan 1,4-Alpha-maltohydrolase Secretion in Pichia pastoris.Applied biochemistry and biotechnology · 2025Article
- Biodiesel Production from Waste Oil Through Efficient Enzymatic Synthesis Using Yarrowia lipolytica Lipase 2 in the Presence of Glucose, β-Cyclodextrin, or G50.Applied biochemistry and biotechnology · 2025Article
- A self-inducible heterologous protein expression system in3 Biotech · 2024Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Lipase B from Candida antarctica (CALB) is an important biocatalyst with many potential applications. However, original CALB is usually with lower enzyme activity and also costly to produce from Candida antarctica; hence, it is often necessary to prepare recombinant CALB through gene heterologous expression. In this research, seven promoters and five signal peptides were compared respectively for expressing codon-optimized CALB in Pichia pastoris, and then recombinant P. pastoris containing 3 copies of calb gene were obtained by screening with high concentrations of antibiotics under the condition of the optimal combination. In a 1.3-L bioreactor, the maximum CALB activity and total protein content reached 444.46 ± 18.81 U/mL and 5.41 ± 0.1 g/L, respectively, after about 9 days of incubation in FM22 medium, which were 34 times and 20 times higher than the initial strains, respectively. In addition, the obtained CALB was used to catalyze the transesterification of acidified gutter oil with methanol, suggesting a promising pathway to convert waste or low quality of bio-oil feedstocks with high amount of free fatty acids into biodiesel by using recombinant CALB as catalyst. The results can provide with a good reference for efficient expression of CALB and enhancing lipase production in P. pastoris. It is supposed to bring with new possibility for the bio-production of other valuable proteins.
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