ArticleClinical oral investigations2023
Amplicon-based next-generation sequencing for comparative analysis of root canal microbiome of teeth with primary and persistent/secondary endodontic infections.
Article in Clinical oral investigations, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 18 citations in OpenAlex.
- Is apical periodontitis a matter of microbial diversity or time? A scoping review.Head & face medicine · 2026Article
- Distinct bacteria profiles in primary and secondary/persistent endodontic infections: a 16S rRNA gene sequencing study.BMC oral health · 2026Article
- Profiling of microbes associated with chronic irreversible pulpitis using metagenomic next-generation sequencing.BMC oral health · 2025Article
- Exploring Relationships Within the Microbiome of Root Canal Infections and the Influence of Associated Clinical Parameters.International endodontic journal · 2025Article
- Comparative Analysis of Root Canal Microbiota in Patients with Diabetes and Systemically Healthy Individuals: A Pilot Next-Generation Sequencing Study.Journal of clinical medicine · 2025Article
- In situ imaging and microbiome analysis of calculus-like deposits at the root apex: A case report of refractory apical periodontitis.International endodontic journal · 2025Article
- Chronic Heat Stress Can Induce Conjugation of a NovelAntibiotics (Basel, Switzerland) · 2025Article
- Association between root canals and gingival sulci microbiota in secondary and persistent endodontic infections.Scientific reports · 2025Article
- Microbiome differences between trauma- and caries-derived periapical lesions using next-generation sequencing.Journal of oral microbiology · 2025Article
- Effects of Sequential Antimicrobial Phases on Root Canal Microbiome Dynamics in Two-Visit Treatment of Primary Apical Periodontitis: A Longitudinal Experimental Study.Life (Basel, Switzerland) · 2024Article
- Odontogenic infections in the antibiotic era: approach to diagnosis, management, and prevention.Infection · 2024Review
- The cause and effect of gut microbiota in development of inflammatory disorders of the breast.European journal of medical research · 2023Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
objectivesTo compare the root canal microbiome profiles of primary and persistent/secondary infections using high-throughput sequencing with the help of a reliable bioinformatics algorithm. MATERIALS AND
methodsRoot canal samples of 10 teeth in the primary endodontic infection (PEI) group and 10 teeth in the persistent/secondary endodontic infection (SEI) group were included resulting in a total of 20 samples. After DNA extraction from the samples, sequencing was performed on the Illumina MiSeq platform. Pair-end Illumina reads were imported to QIIME 2; amplicon sequence variants (ASVs) generated by DADA2 were mapped to GreenGenes database. Weighted UniFrac distances were calculated and principal coordinates analysis (PCoA) was used to compare beta diversity patterns. The multiple response permutation procedure (MRPP), the analysis of similarities (ANOSIM), and permutational multivariate analysis of variance (adonis) were conducted for testing group differences. Linear discriminant analysis effect size (LEfSe) analysis was utilized to identify differentially abundant taxa between the groups. The linear discriminant analysis (LDA) score threshold was set to 4.0.
resultsWithin the Gram-negative facultative anaerobic Gammaproteobacteria class outgroup, two orders (Pasteurellales, Vibrionales) and two families (Pasteurellaceae, Vibrionaceae) were significantly more abundant in the PEI group, whereas Gram-positive bacteria, Actinomycetales order, and Gram-positive anaerobic taxa, one genus (Olsenella) and one species (Olsenella uli), were identified as significantly more abundant in the SEI group.
conclusionsA few taxa were differentially abundant within either the PEI or SEI group. CLINICAL RELEVANCE: Reliable bioinformatic tools are needed to define microbial profiles of endodontic infections. Based on a limited number of samples, no distinct variation was determined between the bacterial diversity of initial and recurrent endodontic infections.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.