Evidence map›Paper›PMID 36722888›Full record

ArticleThe Analyst2023

Single-tube one-step gel-based RT-RPA/PCR for highly sensitive molecular detection of HIV.

Naoki Uno, Ziyue Li, Changchun Liu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Analyst, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Naoki UnoDepartment of Biomedical Engineering, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030, USA. chaliu@uchc.edu.ORCID http://orcid.org/0000-0003-4138-7312
Ziyue LiDepartment of Biomedical Engineering, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030, USA. chaliu@uchc.edu.ORCID http://orcid.org/0000-0002-5040-6696
Changchun LiuDepartment of Biomedical Engineering, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030, USA. chaliu@uchc.edu.ORCID http://orcid.org/0000-0002-4931-986X

Funding

Asymmetric CRISPR Approach for Nucleic Acid QuantificationR01EB023607 · NIBIB · UNIVERSITY OF PENNSYLVANIA · PI Changchun Liu · 2017 to 2026
$2.4M
Self-Powered Sample Concentrating and CRISPR-based Biosensing for Moile HIV-1 RNA DetectionR61AI154642 · NIAID · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · PI LIU, CHANGCHUN · 2020 to 2022
$1.4M
NIAID NIH HHS R61 AI154642NIBIB NIH HHS R01 EB023607
6 · The paper itself

Abstract

We developed a single-tube one-step gel-based reverse transcription-recombinase polymerase amplification (RT-RPA)/polymerase chain reaction (PCR) (termed "SOG RT-RPA/PCR") to detect the human immunodeficiency virus (HIV). To improve the assay sensitivity, the RNA template is pre-amplified by RT-RPA prior to PCR. To simplify the detection process and shorten the assay time, we embedded PCR reagents into agarose gel, constructing it to physically separate the reagents from the RT-RPA reaction solution in a single tube. Due to the thermodynamic properties of agarose, the RT-RPA reaction first occurs independently on top of the PCR gel at a low temperature (

Indexed as

HIV InfectionsNucleic Acid Amplification TechniquesHIVHumansReal-Time Polymerase Chain ReactionRecombinasesReverse TranscriptionRNA, ViralSensitivity and SpecificitySepharoseRecombinasesRNA, ViralSepharose

Identifiers

PMID36722888
PMCPMC9928874

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.