Evidence map›Paper›PMID 36721258›Full record

ArticleJournal of neuroinflammation2023

Plasma derived extracellular vesicle biomarkers of microglia activation in an experimental stroke model.

A D Roseborough, S J Myers, R Khazaee, Y Zhu, L Zhao, E Iorio, F M Elahi, S H Pasternak, S N Whitehead

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of neuroinflammation, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Review
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  5. Article
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  7. Observational
  8. Article
  9. Increased circulating TREM2Brain, behavior, & immunity - health · 2026
    Article
  10. Review
  11. Article
  12. Review
  13. Article
  14. Extracellular RNAs as Messengers and Early Biomarkers in Neurodegeneration.International journal of molecular sciences · 2025
    Review
  15. Article
  16. Article
  17. Article
  18. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

A D RoseboroughVulnerable Brain Laboratory, Department of Anatomy and Cell Biology, The Schulich School of Medicine and Dentistry, The University of Western Ontario, 458 Medical Sciences Building, ON, N6A 3K, London, Canada.
S J MyersVulnerable Brain Laboratory, Department of Anatomy and Cell Biology, The Schulich School of Medicine and Dentistry, The University of Western Ontario, 458 Medical Sciences Building, ON, N6A 3K, London, Canada.
R KhazaeeBiotron Integrated Microscopy Facility, The University of Western Ontario, London, ON, Canada.
Y ZhuVulnerable Brain Laboratory, Department of Anatomy and Cell Biology, The Schulich School of Medicine and Dentistry, The University of Western Ontario, 458 Medical Sciences Building, ON, N6A 3K, London, Canada.
L ZhaoVulnerable Brain Laboratory, Department of Anatomy and Cell Biology, The Schulich School of Medicine and Dentistry, The University of Western Ontario, 458 Medical Sciences Building, ON, N6A 3K, London, Canada.
E IorioWeill Institute for Neurosciences, University of California San Francisco, San Francisco, CA, USA.
F M ElahiWeill Institute for Neurosciences, University of California San Francisco, San Francisco, CA, USA.
S H PasternakIcahn School of Medicine at Mount Sinai, New York, USA.
S N WhiteheadVulnerable Brain Laboratory, Department of Anatomy and Cell Biology, The Schulich School of Medicine and Dentistry, The University of Western Ontario, 458 Medical Sciences Building, ON, N6A 3K, London, Canada. shawn.whitehead@schulich.uwo.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Chronic microglia activation post-stroke is associated with worse neurological and cognitive outcomes. However, measurement of microglia activation in vivo is currently limited. Plasma derived extracellular vesicles (EVs) are cell-specific indicators that may allow for non-invasive measurement of microglia phenotype. The aim of this study was to identify activation-state specific microglia EVs (MEVs) in vitro followed by validation in an experimental stroke model. Following pro-inflammatory activation, MEVs contain the microglia protein TMEM119 alongside increased expression of the Toll-like receptor 4 co-receptor CD14. Immunoprecipitation followed by fluorescent nanoparticle tracking analysis (ONI Nanoimager) was used to confirm the isolation of TMEM119

Indexed as

Extracellular VesiclesMicrogliaStrokeAnimalsBiomarkersCorpus StriatumPhenotypeRatsBiomarkersExtracellular vesicleMicrogliaStroke

Identifiers

PMID36721258
PMCPMC9890769

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.