Evidence map›Paper›PMID 36721171›Full record

ArticleJournal of orthopaedic surgery and research2023

Study on the regulatory effect of Panax notoginseng saponins combined with bone mesenchymal stem cell transplantation on IRAK1/TRAF6-NF-κB pathway in patients with diabetic cutaneous ulcers.

Yuqing Du, Weijian Chen, Youshan Li, Du Liang, Guobin Liu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of orthopaedic surgery and research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.2field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 9 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Yuqing Du *Peripheral Vascular, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China.
Weijian Chen *Guangzhou University of Chinese Medicine, Guangzhou, 510405, Guangdong Province, China.
Youshan LiPeripheral Vascular, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, 100000, China. lys4626@sina.com.
Du LiangGuangzhou University of Chinese Medicine, Guangzhou, 510405, Guangdong Province, China. liangdu_hip@126.com.
Guobin LiuPeripheral Vascular, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China. drlguobin@126.com.
Guangzhou University of Chinese Medicine · CNShuguang Hospital · CNDongzhimen Hospital Affiliated to Beijing University of Chinese Medicine · CN

Funding

Weijian Chen A1-2606-21-429-001Z35Youshan Li 81973849-H2709
6 · The paper itself

Abstract

Panax notoginseng saponins (PNSs) have been found as the major active ingredient of Panax notoginseng (Burkill) F.H.Chen (PN) leaves, which has the effect of reducing inflammatory response, facilitating fibroblast proliferation, as well as promoting angiogenesis. This study aimed to investigate the molecular basis of PNS combined with bone mesenchymal stem cells (BMSCs) for treating diabetic cutaneous ulcers (DCU) and its mechanism of action.

methodsA total of 75 SD rats were selected to make diabetic cutaneous ulcers model. According random number table method, the rats were randomly divided into a control group, a DCU group, a BMSCs group, a PNS group and BMSCs + PNS group. Five groups of rats were given without treatment. After being treated for 7 days, the rats were anesthetized with pentobarbital, and granulation tissue was collected from the central point of the wound. They were used for pathological analysis, Western blot (WB) and polymerase chain reaction (PCR) assays.

resultsThe wound healing area was the largest in the BMSCs + PNS group. HE staining results showed that the PNS + BMSCs group could promote the formation of new epidermis and reduce the infiltration of inflammatory cells. Immunohistochemistry (IHC) results showed that the PNS + BMSCs group could up-regulate the expression of Ki67 protein and cell proliferation. In addition, PNS combined with BMSCs up-regulated the expression of miR-146-5p and down-regulated the expression of IL-1β, IL-6 and TNF-α, IRAK1, TRAF6 and p65 in the NF-κB signaling pathway (p < 0.05).

conclusionsPNS combined with bone mesenchymal stem cell transplantation up-regulated miR-146a-5p targeting and binding to IRAK1/TRAF6, inhibiting the activation of NF-κB pathway, which reduced the inflammatory response of DCU and facilitated the skin healing of DCU. Thus, this study provides a theoretical basis and a novel therapeutic option for the treatment of DFU with PNS combined with BMSCs.

Indexed as

Diabetes ComplicationsDiabetes MellitusMesenchymal Stem Cell TransplantationMicroRNAsPanax notoginsengAnimalsInterleukin-1 Receptor-Associated KinasesNF-kappa BRatsRats, Sprague-DawleyTNF Receptor-Associated Factor 6UlcerInterleukin-1 Receptor-Associated KinasesIRAK1 protein, ratMicroRNAsNF-kappa BTNF Receptor-Associated Factor 6BioinformaticsBone marrow mesenchymal stem cellsDiabetic cutaneous ulcerIRAK1/TRAF6-NF-κB signaling pathwaymiR-146a-5pPanax notoginseng saponins

Identifiers

PMID36721171
PMCPMC9890888
OpenAlexW4318705701

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.