ArticleBMC oral health2023
In vitro comparison of the osteogenic capability of human pulp stem cells on alloplastic, allogeneic, and xenogeneic bone scaffolds.
Article in BMC oral health, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed.
- Evaluation of autogenous tooth bone graft's impact on cellular behaviour and mineralized tissue formation: an in vitro study.BMC oral health · 2026Article
- In Vivo Micro-Computed Tomography for Evaluation of Osteogenic Capability of Dental Pulp Stem Cells Under the Influence of Extracellular Vesicles on Alloplastic and Xenogeneic Bone Scaffolds in Rodent Intrabony Defect Model.Life (Basel, Switzerland) · 2025Article
- Establishing a new periodontitis-like intrabony maxillary defect in rats for investigation on bone regeneration.Scientific reports · 2025Article
- Types of Bone Substitutes and Their Application in Regenerative Medicine: A Systematic Review.Journal of functional biomaterials · 2025Review
- Article
- Use of Plant Extracts in Polymeric Scaffolds in the Regeneration of Mandibular Injuries.Pharmaceutics · 2024Article
- Evaluation of in vitro biocompatibility of human pulp stem cells with allogeneic, alloplastic, and xenogeneic grafts under the influence of extracellular vesicles.Scientific reports · 2023Article
- Dental Pulp Stem Cells for Bone Tissue Engineering: A Literature Review.Stem cells international · 2023Review
Corrections and comments
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Authors and funding
12 authors.
Funding
Abstract
backgroundA rigorous search for alternatives to autogenous bone grafts to avoid invasiveness at the donor site in the treatment of maxillomandibular bone defects. Researchers have used alloplastic, allogeneic, and xenogeneic bone graft substitutes in clinical studies with varying degrees of success, although their in vitro effects on stem cells remain unclear. Dental pulp stem cells (DPSCs) can potentially enhance the bone regeneration of bone graft substitutes. The present in vitro study investigates the osteogenic capability of DPSCs on alloplastic (biphasic calcium phosphate [BCP]), allogeneic (freeze-dried bone allografts [FDBAs]), and xenogeneic (deproteinized bovine bone mineral [DBBM]) bone grafts.
methodsHuman DPSCs were seeded on 0.5 mg/ml, 1 mg/ml, and 2 mg/ml of BCP, FDBA, and DBBM to evaluate the optimal cell growth and cytotoxicity. Scaffolds and cell morphologies were analyzed by scanning electron microscopy (SEM). Calcein AM and cytoskeleton staining were performed to determine cell attachment and proliferation. Alkaline phosphatase (ALP) and osteogenesis-related genes expressions was used to investigate initial osteogenic differentiation.
resultsCytotoxicity assays showed that most viable DPSCs were present at a scaffold concentration of 0.5 mg/ml. The DPSCs on the DBBM scaffold demonstrated a significantly higher proliferation rate of 214.25 ± 16.17 (p < 0.001) cells, enhancing ALP activity level and upregulating of osteogenesis-related genes compared with other two scaffolds.
conclusionDBBP scaffold led to extremely high cell viability, but also promoted proliferation, attachment, and enhanced the osteogenic differentiation capacity of DPSCs, which hold great potential for bone regeneration treatment; however, further studies are necessary.
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