Evidence map›Paper›PMID 36720908›Full record

ArticleScientific reports2023

Rapid clonal identification of biallelic CRISPR/Cas9 knock-ins using SNEAK PEEC.

Sameer Singh, Anoosha Banerjee, Arnaud Vanden Broeck, Sebastian Klinge

Abstract read
In one paragraph

Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. A Manual for Genome and Transcriptome Engineering.IEEE reviews in biomedical engineering · 2025
    Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Sameer SinghLaboratory of Protein and Nucleic Acid Chemistry, The Rockefeller University, New York, NY, 10065, USA.
Anoosha BanerjeeLaboratory of Protein and Nucleic Acid Chemistry, The Rockefeller University, New York, NY, 10065, USA.
Arnaud Vanden BroeckLaboratory of Protein and Nucleic Acid Chemistry, The Rockefeller University, New York, NY, 10065, USA.
Sebastian KlingeLaboratory of Protein and Nucleic Acid Chemistry, The Rockefeller University, New York, NY, 10065, USA. klinge@rockefeller.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

One of the challenges faced by current CRISPR/Cas9 editing strategies is the difficulty in rapidly selecting clonal populations of biallelically edited cells. Here we present Surface engiNeered fluorEscence Assisted Kit with Protein Epitope Enhanced Capture (SNEAK PEEC), a platform that combines human genome editing with cell-surface display, which enables the direct identification of biallelically edited clones with minimal screening.

Indexed as

CRISPR-Cas SystemsGene EditingCell MembraneClone CellsEpitopesHumansEpitopes

Identifiers

PMID36720908
PMCPMC9889345

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.