Evidence map›Paper›PMID 36720795›Full record

ArticleMolecular biology reports2023

Polymorphisms and gene expression of metalloproteinases and their inhibitors associated with cerebral ischemic stroke in young patients with sickle cell anemia.

Ó do Kleyton Palmeira, Ana Karla da Silva Freire, Débora Nascimento de Nóbrega, Roberta Dos Santos Souza, Isabela Cristina Cordeiro Farias, Taciana Furtado de Mendonça Belmont, Andreia Soares da Silva, Gabriela da Silva Arcanjo, Aderson da Silva Araujo, Ana Cláudia Mendonça Dos Anjos and 5 more

Abstract read
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In one paragraph

Article in Molecular biology reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed, 1 pooled it
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 1 synthesis or guideline pooled it, 4 citations in OpenAlex.

  1. Pooled it
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 4 institutions in 1 country.

Ó do Kleyton PalmeiraInstituto Aggeu Magalhães Research Center - IAM-FIOCRUZ-PE, Av. Professor Moraes Rego, S/N, Recife, PE, 50.740-465, Brazil.ORCID http://orcid.org/0000-0002-5271-879X
Ana Karla da Silva FreireInstitute of Biological Sciences and Faculty of Medical Sciences, University of Pernambuco, Recife, PE, Brazil.ORCID http://orcid.org/0000-0003-0338-9536
Débora Nascimento de NóbregaInstituto Aggeu Magalhães Research Center - IAM-FIOCRUZ-PE, Av. Professor Moraes Rego, S/N, Recife, PE, 50.740-465, Brazil.ORCID http://orcid.org/0000-0002-1181-5542
Roberta Dos Santos SouzaInstituto Aggeu Magalhães Research Center - IAM-FIOCRUZ-PE, Av. Professor Moraes Rego, S/N, Recife, PE, 50.740-465, Brazil.ORCID http://orcid.org/0000-0003-3999-3284
Isabela Cristina Cordeiro FariasInstitute of Biological Sciences and Faculty of Medical Sciences, University of Pernambuco, Recife, PE, Brazil.ORCID http://orcid.org/0000-0003-4550-2998
Taciana Furtado de Mendonça BelmontInstitute of Biological Sciences and Faculty of Medical Sciences, University of Pernambuco, Recife, PE, Brazil.ORCID http://orcid.org/0000-0001-7332-1962
Andreia Soares da SilvaInstitute of Biological Sciences and Faculty of Medical Sciences, University of Pernambuco, Recife, PE, Brazil.ORCID http://orcid.org/0000-0003-1600-6899
Gabriela da Silva ArcanjoDepartment of Biological Sciences, Federal University of Pernambuco, Recife, PE, Brazil.ORCID http://orcid.org/0000-0002-1736-0227
Aderson da Silva AraujoHematology and Hemotherapy Foundation of Pernambuco - HEMOPE, Recife, Brazil.ORCID http://orcid.org/0000-0003-1733-5053
Ana Cláudia Mendonça Dos AnjosDepartment of Biological Sciences, Federal University of Pernambuco, Recife, PE, Brazil.ORCID http://orcid.org/0000-0003-4206-4347
Antônio Roberto Lucena de AraujoDepartment of Biological Sciences, Federal University of Pernambuco, Recife, PE, Brazil.ORCID http://orcid.org/0000-0003-1694-8958
Marcos André Cavalcanti BezerraDepartment of Biological Sciences, Federal University of Pernambuco, Recife, PE, Brazil.ORCID http://orcid.org/0000-0002-0175-6147
Patricia Muniz Mendes Freire de MouraInstitute of Biological Sciences and Faculty of Medical Sciences, University of Pernambuco, Recife, PE, Brazil.ORCID http://orcid.org/0000-0003-4259-388X
Maria do Socorro Mendonça CavalcantiInstitute of Biological Sciences and Faculty of Medical Sciences, University of Pernambuco, Recife, PE, Brazil.ORCID http://orcid.org/0000-0001-6235-2739
Luydson Richardson Silva VasconcelosInstituto Aggeu Magalhães Research Center - IAM-FIOCRUZ-PE, Av. Professor Moraes Rego, S/N, Recife, PE, 50.740-465, Brazil. luydson.vasconcelos@fiocruz.br.ORCID http://orcid.org/0000-0002-1625-3358
Universidade de Pernambuco · BRFundação Oswaldo Cruz · BRUniversidade Federal de Pernambuco · BRFundação Hemopa · BR

Funding

Capes Code 001
6 · The paper itself

Abstract

backgroundSickle cell anemia (SCA) is a genetic disease with great clinical heterogeneity and few viable strategies for treatment; hydroxyurea (HU) is the only widely used drug. Thus, the study of single nucleotide polymorphisms (SNPs) and the gene expression of MMPs 1, 2, 9, 7 and TIMPs 1 and 2, which are involved in the regulation of extracellular matrix, inflammation, and neuropathies, may provide further insights into the pathophysiology of the disease and elucidate biomarkers and molecules as potential therapeutic targets for patients with SCA. METHODS AND

resultsWe evaluated 251 young individuals with SCA from northeastern Brazil. The groups were divided according to vaso-occlusive crisis (VOC) and cerebrovascular disease (CVD), compared to control individuals. SNP detection and gene expression assays were performed by real-time PCR, TaqMan system®. Both the expression levels of MMP1 gene, and the SNP MMP1-1607 1G/2G were associated with the risk of cerebral ischemic stroke (IS), and the expression of MMP1 was also associated with a higher frequency of VOC/year. Expression levels of MMP7, TIMP1, and TIMP2 were increased in patients conditioned to IS. The SNP 372T>C (rs4898) TIMP1 T alleles were more frequent in patients with > 5 VOC events/year. The SNP rs17576 of MMP9 showed differences in gene expression levels; it was increased in the genotypes AG, and AG+GG.

conclusionThe findings of this study, the SNPs, and expression provide initial support for understanding the role of MMPs-TIMPs in the pathophysiology of SCA in young patients.

Indexed as

Anemia, Sickle CellIschemic StrokeStrokeVolatile Organic CompoundsGene ExpressionHumansIschemiaMatrix Metalloproteinase 1Matrix MetalloproteinasesPolymorphism, Single NucleotideMatrix Metalloproteinase 1Matrix MetalloproteinasesVolatile Organic CompoundsIschemic strokeMetalloproteinasesSickle cell anemiaVaso-occlusion crisis

Identifiers

PMID36720795
OpenAlexW4318754159

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.