ArticleCurrent biology : CB2023
Microtubule nucleation from the fibrous corona by LIC1-pericentrin promotes chromosome congression.
Article in Current biology : CB, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 29 citations in OpenAlex.
- Mitotic error correction and the spindle assembly checkpoint: a tension-filled relationship.Cell cycle (Georgetown, Tex.) · 2026Review
- Structure and function of the outer kinetochore.Nature reviews. Molecular cell biology · 2026Review
- The KLF4-inflammatory pathway is associated with paraquat-induced pulmonary injury and early pro-fibrotic responses.Biochemistry and biophysics reports · 2026Article
- Outer kinetochore proteins form linear elements to regulate vesicle transport.Journal of cell science · 2026Article
- RSRC2 is a novel RNA-binding protein that safeguards mitotic fidelity by interacting with the lncRNA C1QTNF1-AS1.Nucleic acids research · 2026Article
- The kinetochore corona orchestrates chromosome congression through transient microtubule interactions.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Countervailing effects of cyclin isoforms A and B during mitosis.bioRxiv : the preprint server for biology · 2026Article
- The role of chromosome/kinetochore-driven microtubule generation in Drosophila spindle assembly.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Review
- PLK4: Master Regulator of Centriole Duplication and Its Therapeutic Potential.Cytoskeleton (Hoboken, N.J.) · 2025Review
- CENP-E initiates chromosome congression by opposing Aurora kinases to promote end-on attachments.Nature communications · 2025Article
- Actin organizes chromosomes and microtubules to ensure mitotic fidelity in the preimplantation embryo.Science (New York, N.Y.) · 2025Article
- Article
- Fibrous corona is reduced in cancer cell lines that attenuate microtubule nucleation from kinetochores.Cancer science · 2025Article
- Distinct checkpoint and homolog biorientation pathways regulate meiosis I in Drosophila oocytes.PLoS genetics · 2025Article
- Distinct checkpoint and homolog biorientation pathways regulate meiosis I inbioRxiv : the preprint server for biology · 2024Article
- Centrosome age breaks spindle size symmetry even in cells thought to divide symmetrically.The Journal of cell biology · 2024Article
- Review
- A farnesyl-dependent structural role for CENP-E in expansion of the fibrous corona.The Journal of cell biology · 2024Article
- Securin Regulates the Spatiotemporal Dynamics of Separase.bioRxiv : the preprint server for biology · 2023Article
- RZZ-Spindly and CENP-E form an integrated platform to recruit dynein to the kinetochore corona.The EMBO journal · 2023Article
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Error-free chromosome segregation in mitosis and meiosis relies on the assembly of a microtubule-based spindle that interacts with kinetochores to guide chromosomes to the cell equator before segregation in anaphase. Microtubules sprout from nucleation sites such as centrosomes, but kinetochores can also promote microtubule formation. It is unclear, however, how kinetochore-derived microtubules are generated and what their role is in chromosome segregation. Here, we show that the transient outer-kinetochore meshwork known as the fibrous corona serves as an autonomous microtubule nucleation platform. The fibrous corona is essential for the nucleation of kinetochore-derived microtubules, and when dissociated from the core kinetochore, it retains microtubule nucleation capacity. Nucleation relies on a fibrous-corona-bound pool of the LIC1 subunit of the dynein motor complex, which interacts with the γ-tubulin-tethering protein pericentrin (PCNT). PCNT is essential for microtubule nucleation from fibrous coronas, and in centrosome-depleted cells, where nearly all mitotic nucleation occurs at fibrous coronas, chromosome congression is fully dependent on PCNT. We further show that chromosomes in bovine oocytes, which naturally lack centrosomes, have highly expanded fibrous coronas that drive chromosome-derived microtubule nucleation. Preventing fibrous corona expansion in these cells impairs chromosome congression and causes spindle assembly defects. Our results show that fibrous coronas are autonomous microtubule-organizing centers that are important for spindle assembly, which may be especially relevant in acentrosomal cells such as oocytes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.