ArticlePLoS biology2023
Multisite imaging of neural activity using a genetically encoded calcium sensor in the honey bee.
Article in PLoS biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 27 citations in OpenAlex.
- Emerging tools to advance neuroethology in butterflies and moths.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2026Review
- Form informs function: innervation patterns of projection neurons correspond to response profiles of mushroom body output neurons during olfactory-visual integration.Frontiers in physiology · 2026Article
- Identification of two odorant receptors tuned to alarm pheromone compounds in the honey bee Apis mellifera.Communications biology · 2025Article
- The fru gene specifies male cooperative behaviors in honeybee colonies.Nature communications · 2025Article
- Simultaneous recording of spikes and calcium signals in odor-evoked responses ofJournal of neurogenetics · 2025Article
- In Vivo Two-Photon Imaging of the Olfactory System in Insects.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Temporal effects of sugar intake on fly local search and honey bee dance behaviour.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2024Article
- A perspective on neuroethology: what the past teaches us about the future of neuroethology.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2024Review
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- Fabrication and Validation of Sub-Cellular Carbon Fiber Electrodes.IEEE transactions on neural systems and rehabilitation engineering : a publication of the IEEE Engineering in Medicine and Biology Society · 2024Article
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Corrections and comments
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Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Understanding of the neural bases for complex behaviors in Hymenoptera insect species has been limited by a lack of tools that allow measuring neuronal activity simultaneously in different brain regions. Here, we developed the first pan-neuronal genetic driver in a Hymenopteran model organism, the honey bee, and expressed the calcium indicator GCaMP6f under the control of the honey bee synapsin promoter. We show that GCaMP6f is widely expressed in the honey bee brain, allowing to record neural activity from multiple brain regions. To assess the power of this tool, we focused on the olfactory system, recording simultaneous responses from the antennal lobe, and from the more poorly investigated lateral horn (LH) and mushroom body (MB) calyces. Neural responses to 16 distinct odorants demonstrate that odorant quality (chemical structure) and quantity are faithfully encoded in the honey bee antennal lobe. In contrast, odor coding in the LH departs from this simple physico-chemical coding, supporting the role of this structure in coding the biological value of odorants. We further demonstrate robust neural responses to several bee pheromone odorants, key drivers of social behavior, in the LH. Combined, these brain recordings represent the first use of a neurogenetic tool for recording large-scale neural activity in a eusocial insect and will be of utility in assessing the neural underpinnings of olfactory and other sensory modalities and of social behaviors and cognitive abilities.
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