Evidence map›Paper›PMID 36718777›Full record

ArticleEMBO reports2023

ABCB-mediated shootward auxin transport feeds into the root clock.

Jian Chen, Yangjie Hu, Pengchao Hao, Tashi Tsering, Jian Xia, Yuqin Zhang, Ohad Roth, Maria F Njo, Lieven Sterck, Yun Hu and 6 more

Open access · bronzeAbstract read
In one paragraph

Article in EMBO reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
12.7field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 32 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Review
  5. Article
  6. Article
  7. Review
  8. Review
  9. Article
  10. Auxin and abiotic stress responses.Journal of experimental botany · 2023
    Review
  11. Article
  12. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 4 institutions in 5 countries.

Jian Chen *Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium.ORCID 0000-0001-8238-878X
Yangjie Hu *School of Plant Sciences and Food Security, Tel-Aviv University, Tel-Aviv, Israel.ORCID 0000-0002-0910-6384
Pengchao HaoDepartment of Biology, University of Fribourg, Fribourg, Switzerland.ORCID 0000-0002-7150-5036
Tashi TseringDepartment of Biology, University of Fribourg, Fribourg, Switzerland.
Jian XiaDepartment of Biology, University of Fribourg, Fribourg, Switzerland.ORCID 0000-0001-9294-7960
Yuqin ZhangSchool of Plant Sciences and Food Security, Tel-Aviv University, Tel-Aviv, Israel.
Ohad RothSchool of Plant Sciences and Food Security, Tel-Aviv University, Tel-Aviv, Israel.ORCID 0000-0001-7919-3180
Maria F NjoDepartment of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium.ORCID 0000-0001-7285-5954
Lieven SterckDepartment of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium.ORCID 0000-0001-7116-4000
Yun HuSection of Cell and Developmental Biology, University of California San Diego, La Jolla, CA, USA.
Yunde ZhaoSection of Cell and Developmental Biology, University of California San Diego, La Jolla, CA, USA.
Danny GeelenDepartment of Plants and Crops, Ghent University, Ghent, Belgium.ORCID 0000-0001-8105-3937
Markus GeislerDepartment of Biology, University of Fribourg, Fribourg, Switzerland.ORCID 0000-0002-6641-5810
Eilon ShaniSchool of Plant Sciences and Food Security, Tel-Aviv University, Tel-Aviv, Israel.ORCID 0000-0002-3111-4647
Tom Beeckman *Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium.ORCID 0000-0001-8656-2060
Steffen Vanneste *Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium.ORCID 0000-0002-9948-9672
Ghent University · BETel Aviv University · ILUniversity of Fribourg · CHUniversity of California San Diego · US

Funding

China Scholarship Council (CSC)EC ¦ European Research Council (ERC) 757683Fonds Wetenschappelijk Onderzoek (FWO) G002220NPBCSwiss National Funds 31003A-165877/1
6 · The paper itself

Abstract

Although strongly influenced by environmental conditions, lateral root (LR) positioning along the primary root appears to follow obediently an internal spacing mechanism dictated by auxin oscillations that prepattern the primary root, referred to as the root clock. Surprisingly, none of the hitherto characterized PIN- and ABCB-type auxin transporters seem to be involved in this LR prepatterning mechanism. Here, we characterize ABCB15, 16, 17, 18, and 22 (ABCB15-22) as novel auxin-transporting ABCBs. Knock-down and genome editing of this genetically linked group of ABCBs caused strongly reduced LR densities. These phenotypes were correlated with reduced amplitude, but not reduced frequency of the root clock oscillation. High-resolution auxin transport assays and tissue-specific silencing revealed contributions of ABCB15-22 to shootward auxin transport in the lateral root cap (LRC) and epidermis, thereby explaining the reduced auxin oscillation. Jointly, these data support a model in which LRC-derived auxin contributes to the root clock amplitude.

Indexed as

ArabidopsisArabidopsis ProteinsBiological TransportGene Expression Regulation, PlantIndoleacetic AcidsMembrane Transport ProteinsPlant RootsArabidopsis ProteinsIndoleacetic AcidsMembrane Transport ProteinsABCBauxin transportlateral rootroot meristem

Identifiers

PMID36718777
PMCPMC10074126
OpenAlexW4318616219

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.