Evidence map›Paper›PMID 36717690›Full record

ArticleCommunications biology2023

Split luciferase-based assay to detect botulinum neurotoxins using hiPSC-derived motor neurons.

Laurent Cotter, Feifan Yu, Sylvain Roqueviere, Juliette Duchesne de Lamotte, Johannes Krupp, Min Dong, Camille Nicoleau

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Communications biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Laurent CotterIPSEN Innovation, 5 avenue du Canada, 91940, Les Ulis, France.
Feifan YuDepartment of Urology, Boston Children's Hospital, Boston, MA, 02115, USA.
Sylvain RoqueviereIPSEN Innovation, 5 avenue du Canada, 91940, Les Ulis, France.
Juliette Duchesne de LamotteIPSEN Innovation, 5 avenue du Canada, 91940, Les Ulis, France.
Johannes KruppRecherche Servier, 125 chemin de ronde, 78290, Croissy-sur-seine, France.
Min DongDepartment of Urology, Boston Children's Hospital, Boston, MA, 02115, USA. min.dong@childrens.harvard.edu.ORCID 0000-0002-1744-7293
Camille NicoleauIPSEN Innovation, 5 avenue du Canada, 91940, Les Ulis, France. camille.nicoleau@ipsen.com.ORCID 0000-0002-9803-1053

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Botulinum neurotoxins (BoNTs) have been widely used clinically as a muscle relaxant. These toxins target motor neurons and cleave proteins essential for neurotransmitter release like Synaptosomal-associated protein of 25 kDa (SNAP-25). In vitro assays for BoNT testing using rodent cells or immortalized cell lines showed limitations in accuracy and physiological relevance. Here, we report a cell-based assay for detecting SNAP-25-cleaving BoNTs by combining human induced Pluripotent Stem Cells (hiPSC)-derived motor neurons and a luminescent detection system based on split NanoLuc luciferase. This assay is convenient, rapid, free-of-specialized antibodies, with a detection sensitivity of femtomolar concentrations of toxin, and can be used to study the different steps of BoNT intoxication.

Indexed as

Botulinum Toxins, Type AInduced Pluripotent Stem CellsBiological TransportHumansMotor NeuronsBotulinum Toxins, Type A

Identifiers

PMID36717690
PMCPMC9886929

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.